Biochemical characterization of recombinant guaA-encoded guanosine monophosphate synthetase (EC 6.3.5.2) from Mycobacterium tuberculosis H37Rv strain

• Published in: Archives of biochemistry and biophysics Vol. 517; no. 1; pp. 1 - 11
• Main Authors: Franco, Tathyana Mar A., Rostirolla, Diana C., Ducati, Rodrigo G., Lorenzini, Daniel M., Basso, Luiz A., Santos, Diógenes S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2012
• Subjects: adenosine diphosphate; Adenosine Triphosphatases - metabolism; adenosine triphosphate; adults; Amino Acid Sequence; ammonia; ammonium compounds; calorimetry; Carbon-Nitrogen Ligases - chemistry; Carbon-Nitrogen Ligases - genetics; Carbon-Nitrogen Ligases - isolation & purification; Carbon-Nitrogen Ligases - metabolism; Cloning, Molecular; Cooperative kinetics; glutamic acid; Glutaminase - metabolism; glutamine; GMP synthase (glutamine-hydrolysing); guanosine monophosphate; Guanosine monophosphate synthetase; Humans; hydrolysis; Kinetics; Ligands; magnesium; Magnesium - metabolism; mass spectrometry; Models, Molecular; Molecular Sequence Data; Mycobacterium tuberculosis; Mycobacterium tuberculosis - chemistry; Mycobacterium tuberculosis - enzymology; Mycobacterium tuberculosis - genetics; neonates; operon; Protein Binding; Protein function; Protein Multimerization; Protein Structure, Tertiary; Recombinant protein; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Sequence Alignment; Steady-state kinetics; titration; Titrimetry; Tuberculosis; Tuberculosis - microbiology; vaccines; Cooperative kinetics; Tuberculosis; Recombinant protein; Steady-state kinetics; Protein function; Guanosine monophosphate synthetase

[Display omitted] ► The Mycobacterium tuberculosis guaA gene encodes a homodimeric GMP synthetase (MtGMPS). ► The two-domain type MtGMPS exhibits positive homotropic cooperativity for XMP and NH4+. ► Mg2+ saturation curve is sigmoidal and there may be an additional binding site for this metal. ► Act...