Intracellular Macrophage Growth Rates and Cytokine Profiles of Mycobacterium tuberculosis Strains with Different Transmission Dynamics

• Published in: The Journal of infectious diseases Vol. 191; no. 3; pp. 453 - 460
• Main Authors: Theus, Sue A., Cave, M. Donald, Eisenach, Kathleen D.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.02.2005; University of Chicago Press; Oxford University Press
• Subjects: Bacteria; Biological and medical sciences; Cell growth; Cell Line; Cytokines; Cytokines - metabolism; Disease transmission; DNA Transposable Elements - genetics; Epidemiology; Fundamental and applied biological sciences. Psychology; Humans; Infections; Infectious diseases; Interleukin-10 - metabolism; Macrophages; Macrophages - immunology; Macrophages - microbiology; Medical sciences; Microbiology; Mycobacterium tuberculosis; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - growth & development; Mycobacterium tuberculosis - immunology; Oligonucleotides - analysis; Phenotypes; Polymorphism, Restriction Fragment Length; Tuberculosis; Tuberculosis - epidemiology; Tuberculosis - microbiology; Tuberculosis - transmission; Tumor Necrosis Factor-alpha - metabolism; Virulence; Microbiology; Growth rate; Cytokine; Transmission; Strain; Infection; Mycobacterium tuberculosis; Mycobacteriales; Mycobacteriaceae; Bacteria; Actinomycetes; Intracellular; Macrophage
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/425936

Mycobacterium tuberculosis strains associated with IS6110 restriction fragment-length polymorphism (RFLP) pattern clusters and strains demonstrating unique IS6110 RFLP patterns were investigated in interferon-γ-activated THP-1 cells by measurement of binding, intracellular growth rate, and cytokine production. Binding was the same for all strains; however, strains from clusters grew significantly more rapidly than did unique strains. Maximal concentration of tumor necrosis factor (TNF)-α was detected at 2 days after infection, with unique strains eliciting significantly greater amounts than did strains from clusters. Interleukin (IL)-10 levels peaked at 1 day after infection with strains from clusters, whereas they peaked at 5 days after infection with unique strains. Rapid growth demonstrated by strains from clusters was highly correlated with rapid production of IL-10 and suppression of TNF-α in THP-1 cells during the early stages of infection. Characterization of this phenotype will further advance the investigation of virulence factors in M. tuberculosis.