Isolation methods determine human neutrophil responses after stimulation

• Published in: Frontiers in immunology Vol. 14; p. 1301183
• Main Authors: Krémer, Vanessa, Godon, Ophélie, Bruhns, Pierre, Jönsson, Friederike, de Chaisemartin, Luc
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers 24.11.2023; Frontiers Media S.A
• Subjects: functional analysis; human blood; isolation; Life Sciences; neutrophil (PMN); phenotype; neutrophil (PMN); phenotype; human blood; isolation; functional analysis

Studying neutrophils is challenging due to their limited lifespan, inability to proliferate, and resistance to genetic manipulation. Neutrophils can sense various cues, making them susceptible to activation by blood collection techniques, storage conditions, RBC lysis, and the isolation procedure it...