Signal suppression/enhancement in high-performance liquid chromatography tandem mass spectrometry

• Published in: Journal of Chromatography A Vol. 1217; no. 25; pp. 3929 - 3937
• Main Authors: Gosetti, Fabio, Mazzucco, Eleonora, Zampieri, Davide, Gennaro, Maria Carla
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.06.2010; Amsterdam; New York: Elsevier
• Subjects: atmospheric pressure; Calibration; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; high performance liquid chromatography; HPLC–MS; instrumentation; ionization; Mass spectrometry; Matrix effect; physicochemical properties; quantitative analysis; Signal enhancement; Signal suppression; tandem mass spectrometry; Tandem Mass Spectrometry - instrumentation; Tandem Mass Spectrometry - methods; Tandem Mass Spectrometry - standards; HPLC–MS; Signal enhancement; Matrix effect; Signal suppression; Mass spectrometry

The review discusses the pitfalls of the matrix effect in mass spectrometry detection hyphenated to liquid chromatography separation. Matrix effect heavily influences both qualitative and quantitative analyses, giving rise to suppression or enhancement of the signal. As generally recognised, the pre...