Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images

• Published in: Biophysical journal Vol. 115; no. 6; pp. 951 - 956
• Main Authors: Barentine, Andrew E.S., Schroeder, Lena K., Graff, Michael, Baddeley, David, Bewersdorf, Joerg
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.09.2018; The Biophysical Society
• Subjects: Animals; Cell Survival; Chlorocebus aethiops; Computational Tool; COS Cells; Endoplasmic Reticulum - metabolism; Image Processing, Computer-Assisted; Microscopy, Fluorescence - methods; Software

Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of a nonbiological proxy for a point-like object, such as a fluorescent bead. Although appropriate for...