Sensitive and Specific Serodiagnosis of Lyme Disease by Enzyme-Linked Immunosorbent Assay with a Peptide Based on an Immunodominant Conserved Region of Borrelia burgdorferi VlsE
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| Published in | Journal of Clinical Microbiology Vol. 37; no. 12; pp. 3990 - 3996 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Washington, DC
American Society for Microbiology
01.12.1999
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| AbstractList | VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR(6). In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C(6)) with the IR(6) sequence was explored. Sensitivity was assessed with serum samples (n = 210) collected from patients with clinically defined Lyme disease at the acute (early localized or early disseminated disease), convalescent, or late disease phase. The sensitivities for acute-, convalescent-, and late-phase specimens were 74% (29 of 39), 85 to 90% (34 of 40 to 35 of 39), and 100% (59 of 59), respectively. Serum specimens from early neuroborreliosis patients were 95% positive (19 of 20), and those from an additional group of patients with posttreatment Lyme disease syndrome yielded a sensitivity of 62% (8 of 13). To assess the specificity of the peptide ELISA, 77 serum samples from patients with other spirochetal or chronic infections, autoimmune diseases, or neurologic diseases and 99 serum specimens from hospitalized patients in an area where Lyme disease is not endemic were examined. Only two potential false positives from the hospitalized patients were found, and the overall specificity was 99% (174 of 176). Precision, which was assessed with a panel of positive and negative serum specimens arranged in blinded duplicates, was 100%. Four serum samples with very high anti-OspA antibody titers obtained from four monkeys given the OspA vaccine did not react with the C(6) peptide. This simple, sensitive, specific, and precise ELISA may contribute to alleviate some of the remaining problems in Lyme disease serodiagnosis. Because of its synthetic peptide base, it will be inexpensive to manufacture. It also will be applicable to serum specimens from OspA-vaccinated subjects. VlsE, the variable surface antigen of Borrelia burgdorferi , contains an immunodominant conserved region named IR 6 . In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C 6 ) with the IR 6 sequence was explored. Sensitivity was assessed with serum samples ( n = 210) collected from patients with clinically defined Lyme disease at the acute (early localized or early disseminated disease), convalescent, or late disease phase. The sensitivities for acute-, convalescent-, and late-phase specimens were 74% (29 of 39), 85 to 90% (34 of 40 to 35 of 39), and 100% (59 of 59), respectively. Serum specimens from early neuroborreliosis patients were 95% positive (19 of 20), and those from an additional group of patients with posttreatment Lyme disease syndrome yielded a sensitivity of 62% (8 of 13). To assess the specificity of the peptide ELISA, 77 serum samples from patients with other spirochetal or chronic infections, autoimmune diseases, or neurologic diseases and 99 serum specimens from hospitalized patients in an area where Lyme disease is not endemic were examined. Only two potential false positives from the hospitalized patients were found, and the overall specificity was 99% (174 of 176). Precision, which was assessed with a panel of positive and negative serum specimens arranged in blinded duplicates, was 100%. Four serum samples with very high anti-OspA antibody titers obtained from four monkeys given the OspA vaccine did not react with the C 6 peptide. This simple, sensitive, specific, and precise ELISA may contribute to alleviate some of the remaining problems in Lyme disease serodiagnosis. Because of its synthetic peptide base, it will be inexpensive to manufacture. It also will be applicable to serum specimens from OspA-vaccinated subjects. Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR sub(6). In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C sub(6)) with the IR sub(6) sequence was explored. Sensitivity was assessed with serum samples (n = 210) collected from patients with clinically defined Lyme disease at the acute (early localized or early disseminated disease), convalescent, or late disease phase. The sensitivities for acute-, convalescent-, and late-phase specimens were 74% (29 of 39), 85 to 90% (34 of 40 to 35 of 39), and 100% (59 of 59), respectively. Serum specimens from early neuroborreliosis patients were 95% positive (19 of 20), and those from an additional group of patients with posttreatment Lyme disease syndrome yielded a sensitivity of 62% (8 of 13). To assess the specificity of the peptide ELISA, 77 serum samples from patients with other spirochetal or chronic infections, autoimmune diseases, or neurologic diseases and 99 serum specimens from hospitalized patients in an area where Lyme disease is not endemic were examined. Only two potential false positives from the hospitalized patients were found, and the overall specificity was 99% (174 of 176). Precision, which was assessed with a panel of positive and negative serum specimens arranged in blinded duplicates, was 100%. Four serum samples with very high anti-OspA antibody titers obtained from four monkeys given the OspA vaccine did not react with the C sub(6) peptide. This simple, sensitive, specific, and precise ELISA may contribute to alleviate some of the remaining problems in Lyme disease serodiagnosis. Because of its synthetic peptide base, it will be inexpensive to manufacture. It also will be applicable to serum specimens from OspA-vaccinated subjects. ABSTRACT VlsE, the variable surface antigen of Borrelia burgdorferi , contains an immunodominant conserved region named IR 6 . In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C 6 ) with the IR 6 sequence was explored. Sensitivity was assessed with serum samples ( n = 210) collected from patients with clinically defined Lyme disease at the acute (early localized or early disseminated disease), convalescent, or late disease phase. The sensitivities for acute-, convalescent-, and late-phase specimens were 74% (29 of 39), 85 to 90% (34 of 40 to 35 of 39), and 100% (59 of 59), respectively. Serum specimens from early neuroborreliosis patients were 95% positive (19 of 20), and those from an additional group of patients with posttreatment Lyme disease syndrome yielded a sensitivity of 62% (8 of 13). To assess the specificity of the peptide ELISA, 77 serum samples from patients with other spirochetal or chronic infections, autoimmune diseases, or neurologic diseases and 99 serum specimens from hospitalized patients in an area where Lyme disease is not endemic were examined. Only two potential false positives from the hospitalized patients were found, and the overall specificity was 99% (174 of 176). Precision, which was assessed with a panel of positive and negative serum specimens arranged in blinded duplicates, was 100%. Four serum samples with very high anti-OspA antibody titers obtained from four monkeys given the OspA vaccine did not react with the C 6 peptide. This simple, sensitive, specific, and precise ELISA may contribute to alleviate some of the remaining problems in Lyme disease serodiagnosis. Because of its synthetic peptide base, it will be inexpensive to manufacture. It also will be applicable to serum specimens from OspA-vaccinated subjects. |
| Author | Fang Ting Liang James N. Miller Allen C. Steere Adriana R. Marques Mario T. Philipp Barbara J. B. Johnson |
| AuthorAffiliation | Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433; 1 Division of Rheumatology, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111 2 ; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 3 ; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522 4 ; and Department of Microbiology and Immunology, University of California at Los Angeles, Los Angeles, California 90095 5 |
| AuthorAffiliation_xml | – name: Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433; 1 Division of Rheumatology, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111 2 ; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 3 ; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522 4 ; and Department of Microbiology and Immunology, University of California at Los Angeles, Los Angeles, California 90095 5 |
| Author_xml | – sequence: 1 surname: FANG TING LIANG fullname: FANG TING LIANG organization: Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, United States – sequence: 2 givenname: A. C surname: STEERE fullname: STEERE, A. C organization: Division of Rheumatology, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, United States – sequence: 3 givenname: A. R surname: MARQUES fullname: MARQUES, A. R organization: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States – sequence: 4 givenname: B. J. B surname: JOHNSON fullname: JOHNSON, B. J. B organization: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, United States – sequence: 5 givenname: J. N surname: MILLER fullname: MILLER, J. N organization: Department of Microbiology and Immunology, University of Califomia at Los Angeles, Los Angeles, California 90095, United States – sequence: 6 givenname: M. T surname: PHILIPP fullname: PHILIPP, M. T organization: Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, United States |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1233735$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/10565920$$D View this record in MEDLINE/PubMed |
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| Keywords | Human Lyme disease Borrelia burgdorferi Spirochaetaceae Spirochaetales Peptides Borrelia infection Monkey Serology Lipoprotein Enzyme immunoassay Infection Antigen Vertebrata Experimental disease Conserved sequence Mammalia Animal Bacteriosis Primates Bacteria Diagnosis ELISA assay Spirachaetosis |
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| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Tulane Regional Primate Research Center, Tulane University Medical Center, 18703 Three Rivers Rd., Covington, LA 70433. Phone: (504) 871-6221. Fax: (504) 871-6390. E-mail: philipp@tpc.tulane.edu. |
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Mendeley... VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR(6). In the present study, the diagnostic... ABSTRACT VlsE, the variable surface antigen of Borrelia burgdorferi , contains an immunodominant conserved region named IR 6 . In the present study, the... VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR sub(6). In the present study, the diagnostic... VlsE, the variable surface antigen of Borrelia burgdorferi , contains an immunodominant conserved region named IR 6 . In the present study, the diagnostic... |
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| SubjectTerms | Animals Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Antigens, Bacterial - chemistry Antigens, Bacterial - immunology Antigens, Surface - chemistry Antigens, Surface - immunology Bacterial diseases Bacterial Outer Membrane Proteins - immunology Bacterial Proteins Bacterial Vaccines Bacteriology Biological and medical sciences Borrelia burgdorferi Borrelia burgdorferi Group - immunology Borrelia infections Cross Reactions Enzyme-Linked Immunosorbent Assay - methods Human bacterial diseases Humans Immunodominant Epitopes Infectious diseases Lipoproteins - chemistry Lipoproteins - immunology Lyme Disease - diagnosis Lyme Disease - immunology Lyme Disease - microbiology Macaca mulatta Medical sciences Peptides - chemical synthesis Peptides - chemistry Peptides - immunology Sensitivity and Specificity Serologic Tests Tropical bacterial diseases |
| Title | Sensitive and Specific Serodiagnosis of Lyme Disease by Enzyme-Linked Immunosorbent Assay with a Peptide Based on an Immunodominant Conserved Region of Borrelia burgdorferi VlsE |
| URI | http://jcm.asm.org/content/37/12/3990.abstract https://www.ncbi.nlm.nih.gov/pubmed/10565920 https://search.proquest.com/docview/17350825 https://search.proquest.com/docview/69283594 https://pubmed.ncbi.nlm.nih.gov/PMC85863 |
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