Expression and purification of polioviral proteins in E. coli, and production of antisera as reagents for immunological assays

• Published in: Protein expression and purification Vol. 128; pp. 115 - 122
• Main Authors: Uma, Madala, Rao, P.P., Nagalekshmi, K., Hegde, N.R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2016; Academic Press
• Subjects: Animals; Antibodies, Viral - immunology; Autoinduction; Capsid Proteins - biosynthesis; Capsid Proteins - genetics; Capsid Proteins - immunology; Capsid Proteins - isolation & purification; Cell Line; Cercopithecus aethiops; Escherichia coli; Fibroblasts - immunology; Fibroblasts - virology; Immunoassays; Male; Poliomyelitis - diagnosis; Poliomyelitis - immunology; Poliovirus; Poliovirus - genetics; Poliovirus - immunology; Poliovirus - metabolism; Protein expression; Rabbits; Protein expression; Autoinduction; Poliovirus; Immunoassays

Poliomyelitis, caused by poliovirus, is on the verge of eradication, and the world is preparing to shift from live to inactivated polio vaccine. In view of the requirement of non-infectious reagents, especially protein antigens, for surveillance during the final phase of poliovirus eradication, we h...