Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales

Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale bot...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 362; no. 3; pp. 594 - 600
Main Authors Azuma, Kyoichi, Kobayashi, Masaki, Nakamura, Masahisa, Suzuki, Nobuo, Yashima, Sayaka, Iwamuro, Shawichi, Ikegame, Mika, Yamamoto, Toshio, Hattori, Atsuhiko
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 26.10.2007
Subjects
Acid Phosphatase - biosynthesis
Amino Acid Sequence
Animals
Bone
Bone and Bones - metabolism
Carassius auratus
Cathepsin K
Cathepsins - biosynthesis
Cathepsins - metabolism
Cloning, Molecular
DNA, Complementary - metabolism
Gene Expression Regulation
Goldfish
In Situ Hybridization
Isoenzymes - biosynthesis
Molecular Sequence Data
Morphology
Osteoclasts
Osteoclasts - metabolism
Phylogeny
RNA, Messenger - metabolism
Scale
Sequence
Sequence Homology, Amino Acid
Tartrate-Resistant Acid Phosphatase
Teleostei
Tartrate-resistant acid phosphatase
Cathepsin K
Morphology
Osteoclasts
Scale
Sequence
Bone
Goldfish
Online AccessGet full text

Cover

More Information
Summary:Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.08.010