Effects of prototypical drug-metabolizing enzyme inducers on mRNA expression of housekeeping genes in primary cultures of human and rat hepatocytes

• Published in: Biochemical and biophysical research communications Vol. 346; no. 3; pp. 1033 - 1039
• Main Authors: Nishimura, Masuhiro, Koeda, Akiko, Suzuki, Emako, Shimizu, Takefumi, Kawano, Yuichi, Nakayama, Mitsuo, Satoh, Tetsuo, Narimatsu, Shizuo, Naito, Shinsaku
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.08.2006
• Subjects: Adult; Aged; Animals; Cells, Cultured; Child, Preschool; Dexamethasone - pharmacology; Drug-metabolizing enzyme inducer; Female; Gene Expression Regulation - drug effects; Genes - genetics; Hepatocyte; Hepatocytes - drug effects; Hepatocytes - enzymology; Hepatocytes - metabolism; Housekeeping gene; Human; Humans; Inactivation, Metabolic; Induction; Omeprazole - pharmacology; Pharmaceutical Preparations - metabolism; Rat; Rats; Rats, Sprague-Dawley; Rifampin - pharmacology; RNA, Messenger - genetics; Human; Drug-metabolizing enzyme inducer; Rat; Hepatocyte; Housekeeping gene; Induction

Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-gluc...