Effects of prototypical drug-metabolizing enzyme inducers on mRNA expression of housekeeping genes in primary cultures of human and rat hepatocytes
Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-gluc...
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| Published in | Biochemical and biophysical research communications Vol. 346; no. 3; pp. 1033 - 1039 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier Inc
04.08.2006
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| Abstract | Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-glucuronidase (GUSB), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolylisomerase A (PPIA), TATA box binding protein (TBP), and transferrin receptor (TFRC) in primary cultures of cryopreserved human and rat hepatocytes. The mRNA levels of ACTB, GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in human hepatocytes were constant at all concentrations of inducers. However, the mRNA level of GAPDH relative to HPRT1 in rat hepatocytes was markedly increased by Rif. The mRNA levels of GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in rat hepatocytes were significantly increased by Dex. ACTB and HPRT1 are suitable internal controls for evaluating mRNA expression levels in primary cultures of human and rat hepatocytes after Rif, Dex, or Ome exposure. |
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| AbstractList | Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes beta -actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta -glucuronidase (GUSB), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolylisomerase A (PPIA), TATA box binding protein (TBP), and transferrin receptor (TFRC) in primary cultures of cryopreserved human and rat hepatocytes. The mRNA levels of ACTB, GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in human hepatocytes were constant at all concentrations of inducers. However, the mRNA level of GAPDH relative to HPRT1 in rat hepatocytes was markedly increased by Rif. The mRNA levels of GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in rat hepatocytes were significantly increased by Dex. ACTB and HPRT1 are suitable internal controls for evaluating mRNA expression levels in primary cultures of human and rat hepatocytes after Rif, Dex, or Ome exposure. Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes β-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-glucuronidase (GUSB), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolylisomerase A (PPIA), TATA box binding protein (TBP), and transferrin receptor (TFRC) in primary cultures of cryopreserved human and rat hepatocytes. The mRNA levels of ACTB, GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in human hepatocytes were constant at all concentrations of inducers. However, the mRNA level of GAPDH relative to HPRT1 in rat hepatocytes was markedly increased by Rif. The mRNA levels of GAPDH, GUSB, PPIA, TBP, and TFRC relative to HPRT1 in rat hepatocytes were significantly increased by Dex. ACTB and HPRT1 are suitable internal controls for evaluating mRNA expression levels in primary cultures of human and rat hepatocytes after Rif, Dex, or Ome exposure. |
| Author | Shimizu, Takefumi Nakayama, Mitsuo Suzuki, Emako Satoh, Tetsuo Naito, Shinsaku Narimatsu, Shizuo Nishimura, Masuhiro Koeda, Akiko Kawano, Yuichi |
| Author_xml | – sequence: 1 givenname: Masuhiro surname: Nishimura fullname: Nishimura, Masuhiro email: nisimums@otsukakj.co.jp organization: Department of Drug Metabolism, Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601, Japan – sequence: 2 givenname: Akiko surname: Koeda fullname: Koeda, Akiko organization: Ina Research Inc., Nishiminowa, Ina, Nagano 399-4501, Japan – sequence: 3 givenname: Emako surname: Suzuki fullname: Suzuki, Emako organization: Ina Research Inc., Nishiminowa, Ina, Nagano 399-4501, Japan – sequence: 4 givenname: Takefumi surname: Shimizu fullname: Shimizu, Takefumi organization: Ina Research Inc., Nishiminowa, Ina, Nagano 399-4501, Japan – sequence: 5 givenname: Yuichi surname: Kawano fullname: Kawano, Yuichi organization: Department of Drug Metabolism, Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601, Japan – sequence: 6 givenname: Mitsuo surname: Nakayama fullname: Nakayama, Mitsuo organization: Department of Drug Metabolism, Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601, Japan – sequence: 7 givenname: Tetsuo surname: Satoh fullname: Satoh, Tetsuo organization: Ina Research Inc., Nishiminowa, Ina, Nagano 399-4501, Japan – sequence: 8 givenname: Shizuo surname: Narimatsu fullname: Narimatsu, Shizuo organization: Laboratory of Health Chemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Tsushima-naka, Okayama 700-8530, Japan – sequence: 9 givenname: Shinsaku surname: Naito fullname: Naito, Shinsaku organization: Department of Drug Metabolism, Division of Pharmacology, Drug Safety and Metabolism, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16777070$$D View this record in MEDLINE/PubMed |
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| Keywords | Human Drug-metabolizing enzyme inducer Rat Hepatocyte Housekeeping gene Induction |
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| Title | Effects of prototypical drug-metabolizing enzyme inducers on mRNA expression of housekeeping genes in primary cultures of human and rat hepatocytes |
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