Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles

• Published in: Protein expression and purification Vol. 187; p. 105932
• Main Authors: Zhai, Lukai, Anderson, Dana, Bruckner, Elizabeth, Tumban, Ebenezer
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2021
• Subjects: Amino Acid Sequence; Antibodies, Neutralizing - chemistry; Antibodies, Neutralizing - genetics; Antibodies, Neutralizing - metabolism; Assembly; Bacterial Infections - metabolism; Bacteriophages - metabolism; Capsid Proteins - chemistry; Capsid Proteins - genetics; Capsid Proteins - metabolism; Coat proteins; Escherichia coli; Expression; Gene Expression Regulation; Humans; Protein Binding; Thermophilic bacteriophage; Vaccines, Virus-Like Particle - chemistry; Vaccines, Virus-Like Particle - metabolism; Virus-like particles; Viruses - genetics; Viruses - metabolism; Coat proteins; Expression; Thermophilic bacteriophage; Assembly; Virus-like particles

Virus-like particles (VLPs) have the potential to be used as display platforms to develop vaccines against infectious and non-infectious agents. However, most VLPs used as vaccine display platforms are derived from viruses that infect humans; unfortunately, most humans already have pre-existing anti...