Probe-dependence of competitive fluorescent ligand binding assays to odorant-binding proteins

• Published in: Analytical and bioanalytical chemistry Vol. 412; no. 3; pp. 547 - 554
• Main Authors: Tan, Jiajun, Zaremska, Valeriia, Lim, Sierin, Knoll, Wolfgang, Pelosi, Paolo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2020; Springer; Springer Nature B.V
• Subjects: Affinity; Allosteric properties; Analytical Chemistry; Animals; Assaying; Binding proteins; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Communication; Coordination compounds; Evaluation; Fluorescence; Fluorescent Dyes - metabolism; Fluorescent indicators; Food Science; Investigations; Laboratory Medicine; Ligands; Models, Molecular; Molecular Probes; Monitoring/Environmental Analysis; Odorant-binding protein; Organic chemistry; Organic compounds; Probes; Protein Binding; Proteins; Radioactive tracers; Receptors, Odorant - metabolism; Reliability; Reproducibility of Results; Swine; Fluorescence; Lipocalins; Ligand binding assay; Odorant-binding proteins; Competitive binding

Ligand binding experiments between small chemicals and proteins and the evaluation of dissociation constants of their complexes in competitive binding assays often rely on displacement of reporter probes by the tested ligand. The most widely adopted protocol uses a fluorescent ligand which changes i...