Characterization hiPSC-derived neural progenitor cells and neurons to investigate the role of NOS1AP isoforms in human neuron dendritogenesis

• Published in: Molecular and cellular neuroscience Vol. 109; p. 103562
• Main Authors: Crosta, Christen M., Hernandez, Kristina, Bhattiprolu, Atul K., Fu, Allen Y., Moore, Jennifer C., Clarke, Stephen G., Dudzinski, Natasha R., Brzustowicz, Linda M., Paradiso, Kenneth G., Firestein, Bonnie L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2020
• Subjects: Adaptor Proteins, Signal Transducing - biosynthesis; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - physiology; Antipsychotic medication; Arborization; Cells, Cultured; Clozapine - pharmacology; d-Serine; Dendrites - ultrastructure; Drug Evaluation, Preclinical; Fluphenazine - pharmacology; Gene Expression Regulation - drug effects; Glutamic Acid - physiology; Haloperidol - pharmacology; hiPSC-derived neurons; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Ion Channels - physiology; Morphology; Nerve Tissue Proteins - physiology; Neural Stem Cells - cytology; Neural Stem Cells - metabolism; Neurons - cytology; Neurons - drug effects; Neurons - metabolism; NOS1AP; Oligopeptides - pharmacology; Patch-Clamp Techniques; Protein Isoforms - physiology; Schizophrenia - etiology; Schizophrenia - genetics; Serine - pharmacology; hiPSC-derived neurons; Arborization; d-Serine; Morphology; NOS1AP; Antipsychotic medication

Abnormal dendritic arbor development has been implicated in a number of neurodevelopmental disorders, such as autism and Rett syndrome, and the neuropsychiatric disorder schizophrenia. Postmortem brain samples from subjects with schizophrenia show elevated levels of NOS1AP in the dorsolateral prefro...