Oxidative stress in the mitochondrial matrix underlies ischemia/reperfusion-induced mitochondrial instability

• Published in: The Journal of biological chemistry Vol. 299; no. 1; p. 102780
• Main Authors: Solhjoo, Soroosh, Liu, Ting, Sidor, Agnieszka, Lee, Dong I., O’Rourke, Brian, Steenbergen, Charles
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2023; American Society for Biochemistry and Molecular Biology
• Subjects: Calcium - metabolism; calcium imaging; cardiac monolayers; coverslip-induced ischemia; GSH redox potential; Humans; inner membrane potential oscillations; Ischemia - metabolism; Membrane Potential, Mitochondrial; Mitochondria, Heart - metabolism; Myocytes, Cardiac - metabolism; neonatal rat ventricular myocytes; optical mapping; Oxidative Stress; reactive oxygen species; Reactive Oxygen Species - metabolism; reentry arrhythmias; Reperfusion; reentry arrhythmias; neonatal rat ventricular myocytes; IR; RIRR; cardiac monolayers; TMRM; GSH redox potential; coverslip-induced ischemia; GSH-MEE; reactive oxygen species; ROS; optical mapping; inner membrane potential oscillations; reperfusion; calcium imaging; GSH; NRVM

Ischemia and reperfusion affect multiple elements of cardiomyocyte electrophysiology, especially within the mitochondria. We previously showed that in cardiac monolayers, upon reperfusion after coverslip-induced ischemia, mitochondrial inner membrane potential (ΔΨ) unstably oscillates between polari...