Dual-wavelength excitation for fluorescence-based quantification of zinc protoporphyrin IX and protoporphyrin IX in whole blood

• Published in: Journal of biophotonics Vol. 7; no. 7; pp. 514 - 524
• Main Authors: Hennig, Georg, Gruber, Christian, Vogeser, Michael, Stepp, Herbert, Dittmar, Stephan, Sroka, Ronald, Brittenham, Gary M.
• Format: Journal Article
• Language: English
• Published: Berlin WILEY-VCH Verlag 01.07.2014; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: Algorithms; Anemia, Iron-Deficiency - blood; Anemia, Iron-Deficiency - diagnosis; Biomarkers - blood; Blood; Erythrocytes; Fluorescence; Humans; Liquid chromatography; Microscopy, Fluorescence, Multiphoton - methods; Molecular Imaging - methods; Nutrient deficiency; protoporphyrin IX; Protoporphyrins - blood; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence - methods; spectrum analysis; zinc protoporphyrin; spectrum analysis; zinc protoporphyrin; protoporphyrin IX; fluorescence; blood
• ISSN: 1864-063X; 1864-0648; 1864-0648
• DOI: 10.1002/jbio.201200228

Quantification of erythrocyte zinc protoporphyrin IX (ZnPP) and protoporphyrin IX (PPIX), individually or jointly, is useful for the diagnostic evaluation of iron deficiency, iron‐restricted erythropoiesis, lead exposure, and porphyrias. A method for simultaneous quantification of ZnPP and PPIX in u...