Quantitative imaging of oil storage in developing crop seeds

• Published in: Plant biotechnology journal Vol. 6; no. 1; pp. 31 - 45
• Main Authors: Neuberger, Thomas, Sreenivasulu, Nese, Rokitta, Markus, Rolletschek, Hardy, Göbel, Cornelia, Rutten, Twan, Radchuk, Volodja, Feussner, Ivo, Wobus, Ulrich, Jakob, Peter, Webb, Andrew, Borisjuk, Ljudmilla
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2008; Blackwell Publishing Ltd; Blackwell
• Subjects: analysis; barley; Biological and medical sciences; Biotechnology; chemistry; electron microscopy; Fundamental and applied biological sciences. Psychology; gene expression; Glycine max; Glycine max - chemistry; Glycine max - growth & development; Glycine max - metabolism; growth & development; Hordeum; Hordeum - chemistry; Hordeum - growth & development; Hordeum - metabolism; Hordeum vulgare; image analysis; Liliopsida; lipids; magnetic resonance imaging; Magnetic Resonance Spectroscopy; Magnetic Resonance Spectroscopy - methods; Magnoliopsida; metabolism; methods; oil storage; oils; Plant Oils; Plant Oils - analysis; Plant Oils - metabolism; seed development; seed maturation; seeds; Seeds - chemistry; Seeds - growth & development; Seeds - metabolism; Soybean Oil; Soybean Oil - analysis; Soybean Oil - metabolism; soybeans; tissues; Monocotyledones; magnetic resonance imaging; Seeds; Hordeum vulgare; Vegetable oil; Glycine max; Nuclear magnetic resonance imaging; oil storage; Storage; Leguminosae; Gramineae; Dicotyledones; Angiospermae; seed development; Spermatophyta; Oil plant (vegetal); Quantitative analysis

In this article, we present a tool which allows the rapid and non-invasive detection and quantitative visualization of lipid in living seeds at a variety of stages using frequency-selected magnetic resonance imaging. The method provides quantitative lipid maps with a resolution close to the cellular...