Macelignan protects HepG2 cells against tert-butylhydroperoxide-induced oxidative damage

• Published in: BioFactors (Oxford) Vol. 29; no. 1; pp. 1 - 10
• Main Authors: Sohn, Jong Hee, Han, Kyu Lee, Choo, Jeong Han, Hwang, Jae-Kwan
• Format: Journal Article
• Language: English
• Published: Amsterdam IOS Press 2007
• Subjects: Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Comet Assay; DNA Damage - drug effects; Dose-Response Relationship, Drug; HepG2 cells; Humans; L-Lactate Dehydrogenase - metabolism; Lignans - chemistry; Lignans - pharmacology; Lipid Peroxidation - drug effects; Macelignan; Malondialdehyde - metabolism; Molecular Structure; Myristica fragrans; Oxidation-Reduction - drug effects; oxidative damage; Oxidative Stress - drug effects; Reactive Oxygen Species - metabolism; tert-butylhydroperoxide; tert-Butylhydroperoxide - pharmacology; Time Factors
• ISSN: 0951-6433; 1872-8081
• DOI: 10.1002/biof.5520290101

In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert‐butylhydroperoxide (t‐BHP)‐induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t‐BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose‐dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t‐BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates