Transcriptional analysis of pmeA gene encoding a pectin methylesterase in Xanthomonas campestris pv. campestris

• Published in: Research in microbiology Vol. 162; no. 3; pp. 270 - 278
• Main Authors: Hsiao, Yi-Min, Liu, Yu-Fan, Huang, Yi-Ling, Lee, Pei-Yu
• Format: Journal Article
• Language: English
• Published: France Elsevier SAS 01.04.2011
• Subjects: Amino Acid Sequence; Artificial Gene Fusion; Bacterial Proteins - metabolism; Black rot; Brassicaceae; Carboxylic Ester Hydrolases - biosynthesis; Carboxylic Ester Hydrolases - genetics; Catabolite repression; Cyclic AMP; Cyclic AMP receptor protein-like protein; Enoyl-CoA hydratase; Enoyl-CoA Hydratase - metabolism; exopolysaccharides; Extracellular enzymes; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Bacterial; genes; Genes, Reporter; Molecular Sequence Data; osmolarity; Oxygen; Pectin methylesterase; Pectinesterase; production economics; Promoter- lacZ reporter assay; Sequence Homology, Amino Acid; transcription (genetics); Transcription Factors - metabolism; Transcription initiation; Transcription Initiation Site; Transcriptional regulation; Virulence; Xanthomonas; Xanthomonas campestris; Xanthomonas campestris - enzymology; Xanthomonas campestris - genetics; Xanthomonas campestris pv. campestris; Xanthomonas campestris pv. campestris; Transcriptional regulation; Promoter- lacZ reporter assay; Pectin methylesterase; Cyclic AMP receptor protein-like protein
• ISSN: 0923-2508; 1769-7123; 1769-7123
• DOI: 10.1016/j.resmic.2010.12.002

Exopolysaccharides and several extracellular enzymes of Xanthomonas campestris pv. campestris (Xcc), the causative agent of black rot in crucifers, are virulence determinants. It is known that Clp (cAMP receptor protein-like protein) and RpfF (an enoyl-CoA hydratase homologue required for the synthe...