AglR is required for addition of the final mannose residue of the N-linked glycan decorating the Haloferax volcanii S-layer glycoprotein

• Published in: Biochimica et biophysica acta Vol. 1820; no. 10; pp. 1664 - 1670
• Main Authors: Kaminski, Lina, Guan, Ziqiang, Abu-Qarn, Mehtap, Konrad, Zvia, Eichler, Jerry
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2012
• Subjects: Amino Acid Sequence; Archaea; Archaeal Proteins - genetics; Archaeal Proteins - metabolism; Archaeal Proteins - physiology; biogenesis; bioinformatics; Chromatography, Liquid; Dolichylphosphate-mannose; gene deletion; glycoproteins; Glycosylation; Haloferax volcanii; Haloferax volcanii - genetics; Haloferax volcanii - metabolism; lipopolysaccharides; mannose; Mannose - metabolism; mass spectrometry; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - metabolism; Membrane Glycoproteins - physiology; N-glycosylation; oligosaccharides; Organisms, Genetically Modified; phosphates; plasma membrane; Polysaccharides - chemistry; Polysaccharides - metabolism; post-translational modification; Protein Processing, Post-Translational - genetics; radiolabeling; S-layer glycoprotein; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; ABC; CBB; Haloferax volcanii; DolPP-Man5; Archaea; MS/MS; ER; N-glycosylation; LLO; DolP; DDW; RT-PCR; Dolichylphosphate-mannose; S-layer glycoprotein; LC-ESI/MS; Man; EIC

Recent studies of Haloferax volcanii have begun to elucidate the steps of N-glycosylation in Archaea, where this universal post-translational modification remains poorly described. In Hfx. volcanii, a series of Agl proteins catalyzes the assembly and attachment of a N-linked pentasaccharide to the S...