Direct Whole-Genome Sequencing of Sputum Accurately Identifies Drug-Resistant Mycobacterium tuberculosis Faster than MGIT Culture Sequencing

• Published in: Journal of clinical microbiology Vol. 56; no. 8
• Main Authors: Doyle, Ronan M., Burgess, Carrie, Williams, Rachel, Gorton, Rebecca, Booth, Helen, Brown, James, Bryant, Josephine M., Chan, Jackie, Creer, Dean, Holdstock, Jolyon, Kunst, Heinke, Lozewicz, Stefan, Platt, Gareth, Romero, Erika Yara, Speight, Graham, Tiberi, Simon, Abubakar, Ibrahim, Lipman, Marc, McHugh, Timothy D., Breuer, Judith
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.2018
• Subjects: Antitubercular Agents - pharmacology; Drug Resistance, Bacterial - drug effects; Drug Resistance, Bacterial - genetics; Early Diagnosis; Genome, Bacterial - genetics; Humans; Microbial Sensitivity Tests; Molecular Diagnostic Techniques - methods; Molecular Diagnostic Techniques - standards; Mycobacteriology and Aerobic Actinomycetes; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Sputum - chemistry; Sputum - microbiology; Tuberculosis - diagnosis; Tuberculosis - microbiology; Tuberculosis, Multidrug-Resistant - diagnosis; Tuberculosis, Multidrug-Resistant - microbiology; Whole Genome Sequencing; pathogen DNA enrichment; Mycobacterium tuberculosis; antimicrobial resistance; whole-genome sequencing

The current methods available to diagnose antimicrobial-resistant Mycobacterium tuberculosis infections require a positive culture or only test a limited number of resistance-associated mutations. A rapid accurate identification of antimicrobial resistance enables the prompt initiation of effective...