Isolation of label-free and viable circulating tumour cells (CTCs) from blood samples of cancer patients through a two-step process: negative selection-type immunomagnetic beads and spheroid cell culture-based cell isolation

• Published in: RSC advances Vol. 7; no. 47; pp. 29339 - 29349
• Main Authors: Liao, Chia-Jung, Hsieh, Chia-Hsun, Wang, Hung-Ming, Chou, Wen-Pin, Chiu, Tzu-Keng, Chang, Jyun-Huan, Chao, A.-Ching, Wu, Min-Hsien
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 01.01.2017
• Subjects: Beads; Biotechnology; Blood; Blood circulation; Cancer; Cell culture; Leukocytes; Patients; Purity; Spheroids; Survival; Tumors; Viability

Isolation of high-purity, label-free, and viable circulating tumour cells (CTCs) from cancer patients is crucial for subsequent analyses. To address this issue, a two-step CTC isolation scheme was proposed, wherein a spheroid cell culture was used to further purify viable CTCs after conventional neg...