Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our metho...

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Bibliographic Details
Published inLab on a chip Vol. 2; no. 5; pp. 942 - 948
Main Authors Yu, Jinzhu, Oh, Ki, Moorthi, Sitapriya, Li, Ling, Strey, Helmut H, Schuster, Michael, Luberto, Chiara, Quan, Phenix-Lan, Brouzes, Eric
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 03.03.2020
Subjects
Flow cytometry
Kinases
Phosphorylation
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Summary:We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry. We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. This platform may be broadly applied in single or dual parameter assays to study cell population heterogeneity.
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/c9lc01213c
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Current address: Human Biology Division. Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
ISSN:1473-0197
1473-0189
DOI:10.1039/c9lc01213c