High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 40; no. 5; pp. 1179 - 1186
• Main Authors: Ando, Masayuki, Kaneko, Tohru, Watanabe, Ritsuko, Kikuchi, Satoshi, Goto, Takaaki, Iida, Takashi, Hishinuma, Takanori, Mano, Nariyasu, Goto, Junichi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18.03.2006; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Bile acid; Bile Acids and Salts - blood; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid; Electrospray ionization; Fundamental and applied biological sciences. Psychology; General pharmacology; Glycine - chemistry; Indicators and Reagents; LC–MS/MS; Male; Medical sciences; Pharmacology. Drug treatments; Rat serum; Rats; Rats, Wistar; Reproducibility of Results; Simultaneous determination; Spectrometry, Mass, Electrospray Ionization; Taurine - chemistry; Simultaneous determination; Rat serum; LC–MS/MS; Bile acid; Electrospray ionization; Biological fluid; Rat; Rodentia; Liquid chromatography; Electrospray; LC-MS/MS; Vertebrata; Mammalia; Animal; Mass spectrometry MS/MS; Serum

Sensitive liquid chromatography (LC)/electrospray ionization (ESI) tandem mass spectrometry (MS) can be used to analyze the bile acid composition of rat serum. This method can analyze eight common bile acids and their glycine and taurine conjugates in 100 μl rodent serum by gradient elution on a rev...