Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature

• Published in: Brain, behavior, & immunity. Health Vol. 34; p. 100684
• Main Authors: Wakid, Marina, Almeida, Daniel, Aouabed, Zahia, Rahimian, Reza, Davoli, Maria Antonietta, Yerko, Volodymyr, Leonova-Erko, Elena, Richard, Vincent, Zahedi, René, Borchers, Christoph, Turecki, Gustavo, Mechawar, Naguib
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.12.2023; Elsevier
• Subjects: Blood-Brain Barrier; LC-MS/MS; Microvessels; Neurovascular unit; Postmortem; RNA sequencing; Study Protocol; RNA sequencing; Microvessels; LC-MS/MS; Neurovascular unit; Postmortem; Blood-Brain Barrier

The neurovascular unit, comprised of vascular cell types that collectively regulate cerebral blood flow to meet the needs of coupled neurons, is paramount for the proper function of the central nervous system. The neurovascular unit gatekeeps blood-brain barrier properties, which experiences impairm...