Pre-treatment of cells with pluronic L64 increases DNA transfection mediated by electrotransfer

• Published in: Journal of controlled release Vol. 149; no. 2; pp. 117 - 125
• Main Authors: Wasungu, L., Marty, A.L., Bureau, M.F., Kichler, A., Bessodes, M., Teissie, J., Scherman, D., Rols, M.P., Mignet, N.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 20.01.2011; Elsevier
• Subjects: Animals; Biological and medical sciences; biomedical research; biopharmaceuticals; Cell Membrane Permeability - drug effects; Cell Survival - drug effects; cell viability; CHO Cells; composite polymers; Copolymer; Cricetinae; Cricetulus; DNA - administration & dosage; DNA - genetics; Drug Carriers - chemistry; Drug Carriers - pharmacology; electric field; Electroporation; Electrotransfer; Gene delivery; gene expression; Gene Transfer Techniques; General pharmacology; Genes, Reporter; genetic disorders; Luciferases - genetics; Mechanism; mechanism of action; Medical sciences; muscle tissues; muscles; myocytes; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Plasmids; Poloxamer - chemistry; Poloxamer - pharmacology; pretreatment; secretion; signal transduction; therapeutics; transcription factor NF-kappa B; Transfection; Gene delivery; Electroporation; Copolymer; Electrotransfer; Mechanism; Pharmaceutical technology; Vehicle(excipient); Laxative; Ethylene oxide copolymer; In vitro; Genetic transfer; Polyelectrolyte; Triblock copolymer; Treatment; Transfection; Cyclic ether copolymer; DNA; Poloxamer; Propylene oxide copolymer; Mechanism of action; Gene therapy; Nucleic acid

Gene transfer into muscle cells is a key issue in biomedical research. Indeed, it is important for the development of new therapy for many genetic disorders affecting this tissue and for the use of muscle tissue as a secretion platform of therapeutic proteins. Electrotransfer is a promising method t...