A Genetically Engineered Rotavirus NSP2 Phosphorylation Mutant Impaired in Viroplasm Formation and Replication Shows an Early Interaction between vNSP2 and Cellular Lipid Droplets

• Published in: Journal of virology Vol. 94; no. 15
• Main Authors: Criglar, Jeanette M, Crawford, Sue E, Zhao, Boyang, Smith, Hunter G, Stossi, Fabio, Estes, Mary K
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 16.07.2020
• Subjects: Animals; Cell Line; Cricetinae; Lipid Droplets - metabolism; Lipid Droplets - virology; Phosphorylation; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Rotavirus - physiology; Spotlight; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; Virus Replication - physiology; Virus-Cell Interactions; lipid droplet; replication; perilipin; NSP2; rotavirus; CK1α; PLIN1; protein phosphorylation; viroplasm; NSP5

Many RNA viruses replicate in cytoplasmic compartments (virus factories or viroplasms) composed of viral and cellular proteins, but the mechanisms required for their formation remain largely unknown. Rotavirus (RV) replication in viroplasms requires interactions between virus nonstructural proteins...