Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera

• Published in: Biosensors & bioelectronics Vol. 24; no. 7; pp. 2143 - 2148
• Main Authors: Vaisocherová, Hana, Faca, Vitor M., Taylor, Allen D., Hanash, Samir, Jiang, Shaoyi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.03.2009; Elsevier
• Subjects: ALCAM; Antigens, CD - blood; Biological and medical sciences; Biomarkers, Tumor - blood; Biosensing Techniques - methods; Biotechnology; Blood Chemical Analysis - methods; Blood serum; Cancer biomarkers; CD166; Cell Adhesion Molecules, Neuronal - blood; ELISA; Enzyme-Linked Immunosorbent Assay - methods; Fetal Proteins; Fundamental and applied biological sciences. Psychology; Humans; Neoplasm Proteins - blood; Pancreatic Neoplasms - blood; Pancreatic Neoplasms - diagnosis; Reproducibility of Results; Sensitivity and Specificity; SPR; Surface Plasmon Resonance - methods; Cancer biomarkers; ALCAM; SPR; Blood serum; ELISA; CD166; Human; Biological marker; Method; Malignant tumor; Surface plasmon; Blood; Serum; ELISA assay; Comparative study; Cancer

Surface plasmon resonance (SPR), as a label free method for analysis of various analytes, has significantly advanced in recent years. However, assessment of the performance of SPR compared to label-based immunoassays such as the commonly used multiplexed enzyme-linked immunosorbent assay (ELISA) is...