The Dock and Lock Method: A Novel Platform Technology for Building Multivalent, Multifunctional Structures of Defined Composition with Retained Bioactivity

• Published in: Clinical cancer research Vol. 13; no. 18; pp. 5586s - 5591s
• Main Authors: CHANG, Chien-Hsing, ROSSI, Edmund A, GOLDENBERG, David M
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.09.2007
• Subjects: A-kinase anchoring proteins; Amino Acid Sequence; Animals; Antineoplastic agents; Biological and medical sciences; bispecific antibody; Cyclic AMP-Dependent Protein Kinases - chemistry; Dimerization; Humans; Immunoglobulin Fab Fragments - chemistry; Medical sciences; Models, Molecular; Molecular Sequence Data; multifunctional; multivalent; Pharmacology. Drug treatments; Recombinant Fusion Proteins - chemistry; Sequence Homology, Amino Acid; Composition; Method; Structure; Technology; Biological activity

The idea, approach, and proof-of-concept of the dock and lock (DNL) method, which has the potential for making a large number of bioactive molecules with multivalency and multifunctionality, are reviewed. The key to the DNL method seems to be the judicious application of a pair of distinct protein d...