Characterization of Recombinant Flaviviridae Viruses Possessing a Small Reporter Tag

• Published in: Journal of virology Vol. 92; no. 2
• Main Authors: Tamura, Tomokazu, Fukuhara, Takasuke, Uchida, Takuro, Ono, Chikako, Mori, Hiroyuki, Sato, Asuka, Fauzyah, Yuzy, Okamoto, Toru, Kurosu, Takeshi, Setoh, Yin Xiang, Imamura, Michio, Tautz, Norbert, Sakoda, Yoshihiro, Khromykh, Alexander A., Chayama, Kazuaki, Matsuura, Yoshiharu
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 15.01.2018
• Subjects: Animals; Antiviral Agents - pharmacology; Cell Line; Dose-Response Relationship, Drug; Drug Discovery; Drug Evaluation, Preclinical; Flaviviridae - drug effects; Flaviviridae - genetics; Gene Expression; Genes, Reporter; Genome, Viral; Hepacivirus - genetics; Humans; Mice; Mutagenesis, Insertional; Recombination, Genetic; Virus-Cell Interactions; in vivo dynamics; reporter virus; antiviral screening; Flaviviridae

The family Flaviviridae consists of four genera, Flavivirus , Pestivirus , Pegivirus , and Hepacivirus , and comprises important pathogens of human and animals. Although the construction of recombinant viruses carrying reporter genes encoding fluorescent and bioluminescent proteins has been reported...