Characterization of Recombinant Flaviviridae Viruses Possessing a Small Reporter Tag

• Published in: Journal of virology Vol. 92; no. 2
• Main Authors: Tamura, Tomokazu, Fukuhara, Takasuke, Uchida, Takuro, Ono, Chikako, Mori, Hiroyuki, Sato, Asuka, Fauzyah, Yuzy, Okamoto, Toru, Kurosu, Takeshi, Setoh, Yin Xiang, Imamura, Michio, Tautz, Norbert, Sakoda, Yoshihiro, Khromykh, Alexander A, Chayama, Kazuaki, Matsuura, Yoshiharu
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 15.01.2018
• Subjects: Animals; Antiviral Agents - pharmacology; Cell Line; Dose-Response Relationship, Drug; Drug Discovery; Drug Evaluation, Preclinical; Flaviviridae - drug effects; Flaviviridae - genetics; Gene Expression; Genes, Reporter; Genome, Viral; Hepacivirus - genetics; Humans; Mice; Mutagenesis, Insertional; Recombination, Genetic; Virus-Cell Interactions; in vivo dynamics; reporter virus; antiviral screening; Flaviviridae

The family consists of four genera, , , , and , and comprises important pathogens of human and animals. Although the construction of recombinant viruses carrying reporter genes encoding fluorescent and bioluminescent proteins has been reported, the stable insertion of foreign genes into viral genome...