Detection of antibodies to Trypanosoma cruzi in the South American opossum ( Didelphis marsupialis)
Stocks of Trypanosoma cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild reservoir hosts, were used as sources of diagnostic antigens in enzyme-linked immunosorbent assays for the detection of antibodies to T. cru...
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Published in | Transactions of the Royal Society of Tropical Medicine and Hygiene Vol. 76; no. 1; pp. 29 - 32 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
1982
Royal Society of Tropical Medicine and Hygiene |
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Abstract | Stocks of
Trypanosoma cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild reservoir hosts, were used as sources of diagnostic antigens in enzyme-linked immunosorbent assays for the detection of antibodies to
T. cruzi in
Didelphis marsupialis. Both antigen preparations reacted with antibodies in sera from animals found to be infected by conventional parasitological techniques and also in sera from a proportion of the remaining animals in which it was not possible to detect trypanosomes. |
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AbstractList | Stocks of T. cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild reservoir hosts, were used as sources of diagnostic antigens in enzyme-linked immunosorbent assays for the detection of antibodies to T. cruzi in D. marsupialis . Both antigen preparations reacted with antibodies in sera from animals found to be infected by conventional parasitological techniques and also in sera from a proportion of the remaining animals in which it was not possible to detect trypanosomes. Trypanosoma cruzi, detection of antibodies in Didelphis marsupialis using enzyme-linked immunosorbent assay: Brazil Stocks of Trypanosoma cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild reservoir hosts, were used as sources of diagnostic antigens in enzyme-linked immunosorbent assays for the detection of antibodies to T. cruzi in Didelphis marsupialis. Both antigen preparations reacted with antibodies in sera from animals found to be infected by conventional parasitological techniques and also in sera from a proportion of the remaining animals in which it was not possible to detect trypanosomes. Stocks of Trypanosoma cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild reservoir hosts, were used as sources of diagnostic antigens in enzyme-linked immunosorbent assays for the detection of antibodies to T. cruzi in Didelphis marsupialis. Both antigen preparations reacted with antibodies in sera from animals found to be infected by conventional parasitological techniques and also in sera from a proportion of the remaining animals in which it was not possible to detect trypanosomes. |
Author | Luckins, A.G. Miles, M.A. |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/6805104$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1016/0035-9203(77)90012-8 10.1016/0035-9203(79)90211-6 10.1016/0022-1759(81)90068-5 10.4049/jimmunol.100.4.736 10.1016/0019-2791(70)90036-4 10.4269/ajtmh.1979.28.969 10.1016/0035-9203(81)90194-2 10.1016/0019-2791(71)90395-8 10.1016/0035-9203(80)90251-5 10.1016/S0140-6736(75)91492-0 10.4049/jimmunol.101.6.1207 10.4049/jimmunol.106.4.1043 10.1016/0035-9203(81)90145-0 10.4049/jimmunol.109.1.129 |
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References | Engvall, Perlmann (BIB5) 1972; 109 (BIB1) 1976; 54 Minter-Goedbloed, Franca, Draper (BIB15) 1980; 83 Pain, Surolia (BIB16) 1981; 40 Rowlands, Dudley (BIB17) 1968; 100 Miles (BIB11) 1979 Anthony, Johnson, Souza (BIB3) 1979; 28 Arias, Naiff, Miles, Souza (BIB2) 1981; 75 Miles, Toye, Oswald, Godfrey (BIB12) 1977; 71 Miles, Povoa, Souza, Lainson, Shaw, Ketteridge (BIB14) 1981; 75 Taylor, Burrell (BIB18) 1968; 101 Lainson, Shaw, Fraiha, Miles, Draper (BIB9) 1979; 73 Kronvall, Frommel (BIB8) 1970; 7 Voller, Draper, Bidwell, Bartlett (BIB20) 1975; i Hoare (BIB6) 1972 Kagan, Goldsmith, Zarate-Costaneda, Allain (BIB7) 1978; 12 Miles, Lanham, Souza, Povóa (BIB13) 1980; 74 Voller, Bidwell, Bartlett (BIB19) 1975; 26 Avrameas, Ternynck (BIB4) 1971; 8 Marx, Burrell, Fisher (BIB10) 1971; 106 Rowlands (10.1016/0035-9203(82)90010-4_BIB17) 1968; 100 (10.1016/0035-9203(82)90010-4_BIB1) 1976; 54 Miles (10.1016/0035-9203(82)90010-4_BIB13) 1980; 74 Anthony (10.1016/0035-9203(82)90010-4_BIB3) 1979; 28 Marx (10.1016/0035-9203(82)90010-4_BIB10) 1971; 106 Voller (10.1016/0035-9203(82)90010-4_BIB20) 1975; i Lainson (10.1016/0035-9203(82)90010-4_BIB9) 1979; 73 Miles (10.1016/0035-9203(82)90010-4_BIB14) 1981; 75 Arias (10.1016/0035-9203(82)90010-4_BIB2) 1981; 75 Miles (10.1016/0035-9203(82)90010-4_BIB11) 1979 Kronvall (10.1016/0035-9203(82)90010-4_BIB8) 1970; 7 Avrameas (10.1016/0035-9203(82)90010-4_BIB4) 1971; 8 Engvall (10.1016/0035-9203(82)90010-4_BIB5) 1972; 109 Kagan (10.1016/0035-9203(82)90010-4_BIB7) 1978; 12 Miles (10.1016/0035-9203(82)90010-4_BIB12) 1977; 71 Minter-Goedbloed (10.1016/0035-9203(82)90010-4_BIB15) 1980; 83 Voller (10.1016/0035-9203(82)90010-4_BIB19) 1975; 26 Hoare (10.1016/0035-9203(82)90010-4_BIB6) 1972 Pain (10.1016/0035-9203(82)90010-4_BIB16) 1981; 40 Taylor (10.1016/0035-9203(82)90010-4_BIB18) 1968; 101 |
References_xml | – volume: i start-page: 426 year: 1975 end-page: 429 ident: BIB20 article-title: Microplate enzyme-linked immunosorbent assay for Chagas' disease publication-title: Lancet contributor: fullname: Bartlett – volume: 40 start-page: 219 year: 1981 end-page: 230 ident: BIB16 article-title: Preparation of protein A—peroxidase monoconjugate using a heterobi-functional reagent, and its use in enzyme immunoassays publication-title: Journal of Immunological Methods contributor: fullname: Surolia – volume: 54 start-page: 141 year: 1976 end-page: 147 ident: BIB1 article-title: Parallel evaluation of serological tests applied in African trypanosomiasis: a WHO Collaborative study publication-title: Bulletin of the World Health Organisation – volume: 75 start-page: 667 year: 1981 end-page: 674 ident: BIB14 article-title: Chagas's disease in the Amazon Basin: II. The distribution of publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene contributor: fullname: Ketteridge – volume: 101 start-page: 1207 year: 1968 end-page: 1216 ident: BIB18 article-title: The immunologic response of the North American opossum ( publication-title: Journal of Immunology contributor: fullname: Burrell – year: 1972 ident: BIB6 publication-title: The Trypanosomes of Mammals. 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Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes publication-title: Journal of Immunology contributor: fullname: Perlmann – volume: 74 start-page: 221 year: 1980 end-page: 237 ident: BIB13 article-title: Further enzymic characters of publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene contributor: fullname: Povóa – start-page: 117 year: 1979 end-page: 196 ident: BIB11 article-title: Transmission cycles and the heterogeneity of publication-title: Biology of the Kinetoplastida contributor: fullname: Miles – volume: 12 start-page: 341 year: 1978 end-page: 348 ident: BIB7 article-title: Evaluation of serologic tests for studies on Chagas' disease publication-title: Bulletin of the Pan-American Health Organization contributor: fullname: Allain – volume: 73 start-page: 192 year: 1979 end-page: 204 ident: BIB9 article-title: Chagas's disease in the Amazon Basin: I. publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene contributor: fullname: Draper – volume: 75 start-page: 537 year: 1981 end-page: 541 ident: BIB2 article-title: The opossum publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene contributor: fullname: Souza – volume: 106 start-page: 1043 year: 1971 end-page: 1049 ident: BIB10 article-title: A study of the afferent and efferent limbs of the immune response in opossums publication-title: Journal of Immunology contributor: fullname: Fisher – volume: 100 start-page: 736 year: 1968 end-page: 743 ident: BIB17 article-title: The isolation of immunoglobulins of adult opossums ( publication-title: Journal of Immunology contributor: fullname: Dudley – volume: 28 start-page: 969 year: 1979 end-page: 973 ident: BIB3 article-title: Use of microELISA for quantitating antibody to publication-title: American Journal of Tropical Medicine and Hygiene contributor: fullname: Souza – volume: 83 start-page: 157 year: 1980 end-page: 160 ident: BIB15 article-title: The latex agglutination for publication-title: Journal of Tropical Medicine and Hygiene contributor: fullname: Draper – volume: 26 start-page: 247 year: 1975 end-page: 251 ident: BIB19 article-title: A serological study on human publication-title: Tropenmedizin und Parasitologie contributor: fullname: Bartlett – volume: 71 start-page: 217 year: 1977 end-page: 225 ident: BIB12 article-title: The identification by isoenzyme patterns of two distinct strain-groups of publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene contributor: fullname: Godfrey – volume: 12 start-page: 341 year: 1978 ident: 10.1016/0035-9203(82)90010-4_BIB7 article-title: Evaluation of serologic tests for studies on Chagas' disease publication-title: Bulletin of the Pan-American Health Organization contributor: fullname: Kagan – volume: 71 start-page: 217 year: 1977 ident: 10.1016/0035-9203(82)90010-4_BIB12 article-title: The identification by isoenzyme patterns of two distinct strain-groups of Trypanosoma cruzi, circulating independently in a rural area of Brazil publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene doi: 10.1016/0035-9203(77)90012-8 contributor: fullname: Miles – volume: 73 start-page: 192 year: 1979 ident: 10.1016/0035-9203(82)90010-4_BIB9 article-title: Chagas's disease in the Amazon Basin: I. Trypanosoma cruzi infections in silvatic mammals, triatomine bugs and man, in the State of Pára, north Brazil publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene doi: 10.1016/0035-9203(79)90211-6 contributor: fullname: Lainson – volume: 40 start-page: 219 year: 1981 ident: 10.1016/0035-9203(82)90010-4_BIB16 article-title: Preparation of protein A—peroxidase monoconjugate using a heterobi-functional reagent, and its use in enzyme immunoassays publication-title: Journal of Immunological Methods doi: 10.1016/0022-1759(81)90068-5 contributor: fullname: Pain – volume: 100 start-page: 736 year: 1968 ident: 10.1016/0035-9203(82)90010-4_BIB17 article-title: The isolation of immunoglobulins of adult opossums (Didelphis virginiana) publication-title: Journal of Immunology doi: 10.4049/jimmunol.100.4.736 contributor: fullname: Rowlands – volume: 7 start-page: 124 year: 1970 ident: 10.1016/0035-9203(82)90010-4_BIB8 article-title: Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments publication-title: Immunochemistry doi: 10.1016/0019-2791(70)90036-4 contributor: fullname: Kronvall – volume: 28 start-page: 969 year: 1979 ident: 10.1016/0035-9203(82)90010-4_BIB3 article-title: Use of microELISA for quantitating antibody to Trypanosoma cruzi and Trypanosoma rangeli publication-title: American Journal of Tropical Medicine and Hygiene doi: 10.4269/ajtmh.1979.28.969 contributor: fullname: Anthony – volume: 75 start-page: 537 year: 1981 ident: 10.1016/0035-9203(82)90010-4_BIB2 article-title: The opossum Didelphis marsupialis (Marsupialia: Didelphidiae), as a reservoir-host of Leishmania braziliensis guyanensis in the Amazon Basin of Brazil publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene doi: 10.1016/0035-9203(81)90194-2 contributor: fullname: Arias – year: 1972 ident: 10.1016/0035-9203(82)90010-4_BIB6 contributor: fullname: Hoare – start-page: 117 year: 1979 ident: 10.1016/0035-9203(82)90010-4_BIB11 article-title: Transmission cycles and the heterogeneity of Trypanosoma cruzi contributor: fullname: Miles – volume: 8 start-page: 1175 year: 1971 ident: 10.1016/0035-9203(82)90010-4_BIB4 article-title: Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration publication-title: Immunochemistry doi: 10.1016/0019-2791(71)90395-8 contributor: fullname: Avrameas – volume: 26 start-page: 247 year: 1975 ident: 10.1016/0035-9203(82)90010-4_BIB19 article-title: A serological study on human Trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay publication-title: Tropenmedizin und Parasitologie contributor: fullname: Voller – volume: 74 start-page: 221 year: 1980 ident: 10.1016/0035-9203(82)90010-4_BIB13 article-title: Further enzymic characters of Trypanosoma cruzi and their evaluation for strain identification publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene doi: 10.1016/0035-9203(80)90251-5 contributor: fullname: Miles – volume: i start-page: 426 year: 1975 ident: 10.1016/0035-9203(82)90010-4_BIB20 article-title: Microplate enzyme-linked immunosorbent assay for Chagas' disease publication-title: Lancet doi: 10.1016/S0140-6736(75)91492-0 contributor: fullname: Voller – volume: 83 start-page: 157 year: 1980 ident: 10.1016/0035-9203(82)90010-4_BIB15 article-title: The latex agglutination for Trypanosoma cruzi: unsuitable for testing animals publication-title: Journal of Tropical Medicine and Hygiene contributor: fullname: Minter-Goedbloed – volume: 101 start-page: 1207 year: 1968 ident: 10.1016/0035-9203(82)90010-4_BIB18 article-title: The immunologic response of the North American opossum (Didelphis virginiana) publication-title: Journal of Immunology doi: 10.4049/jimmunol.101.6.1207 contributor: fullname: Taylor – volume: 106 start-page: 1043 year: 1971 ident: 10.1016/0035-9203(82)90010-4_BIB10 article-title: A study of the afferent and efferent limbs of the immune response in opossums publication-title: Journal of Immunology doi: 10.4049/jimmunol.106.4.1043 contributor: fullname: Marx – volume: 75 start-page: 667 year: 1981 ident: 10.1016/0035-9203(82)90010-4_BIB14 article-title: Chagas's disease in the Amazon Basin: II. The distribution of Trypanosoma cruzi zymodemes 1 and 3 in Pará State, north Brazil publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene doi: 10.1016/0035-9203(81)90145-0 contributor: fullname: Miles – volume: 54 start-page: 141 year: 1976 ident: 10.1016/0035-9203(82)90010-4_BIB1 article-title: Parallel evaluation of serological tests applied in African trypanosomiasis: a WHO Collaborative study publication-title: Bulletin of the World Health Organisation – volume: 109 start-page: 129 year: 1972 ident: 10.1016/0035-9203(82)90010-4_BIB5 article-title: Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes publication-title: Journal of Immunology doi: 10.4049/jimmunol.109.1.129 contributor: fullname: Engvall |
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Trypanosoma cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild... Trypanosoma cruzi, detection of antibodies in Didelphis marsupialis using enzyme-linked immunosorbent assay: Brazil Stocks of Trypanosoma cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild... Stocks of T. cruzi belonging to two different zymodemes, one usually associated with silvatic reservoir hosts and the other not normally found in wild... |
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SubjectTerms | Animals Antibodies - analysis Brazil Chagas Disease - immunology Chagas Disease - veterinary Didelphis marsupialis disease reservoirs Enzyme-Linked Immunosorbent Assay enzymes immunity labeling techniques Opossums - immunology Opossums - parasitology serodiagnosis Trypanosoma cruzi Trypanosoma cruzi - immunology |
Title | Detection of antibodies to Trypanosoma cruzi in the South American opossum ( Didelphis marsupialis) |
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