Ubiquitination of Major Histocompatibility Complex II Changes Its Immunological Recognition Structure

• Published in: International journal of molecular sciences Vol. 24; no. 23; p. 17083
• Main Authors: Kozono, Yuko, Kuramochi, Masahiro, Sasaki, Yuji C, Kozono, Haruo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.12.2023
• Subjects: Antigens; B cells; Dendritic Cells; Histocompatibility Antigens Class II - metabolism; LAG-3; Ligases; Lymphocytes; Major Histocompatibility Complex; MHC; Nanocrystals; Peptides; single-molecule biophysics; T cells; Ubiquitin; Ubiquitin - metabolism; ubiquitin ligase; Ubiquitin-Protein Ligases - metabolism; Ubiquitination; Japan; ubiquitin ligase; single-molecule biophysics; MHC; dendritic cells; LAG-3
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms242317083

Ubiquitination is a process that dictates the lifespan of major histocompatibility complex class II (MHC II)/peptide complexes on antigen-presenting cells. This process is tightly controlled by the levels of ubiquitin ligases, and disruptions in the turnover of MHC II can lead to the improper development of CD4+ T cells within the thymus and hinder the formation of regulatory T cells in the peripheral tissue. To investigate the underlying mechanisms, we utilized dendritic cells lacking the Membrane-associated RING-CH (MARCH) I ubiquitin ligase. We discovered that the overexpression of MARCH I decreases the interaction with LAG-3. Moreover, the MHC II molecules tethered with ubiquitin also showed diminished binding to LAG-3. We employed Diffracted X-ray Blinking (DXB), a technique used for single-molecule X-ray imaging, to observe the protein movements on live cells in real time. Our observations indicated that the normal MHC II molecules moved more rapidly across the cell surface compared to those on the MARCH I-deficient dendritic cells or MHC II KR mutants, which is likely a result of ubiquitination. These findings suggest that the signaling from ubiquitinated MHC II to the T cell receptor differs from the non-ubiquitinated forms. It appears that ubiquitinated MHC II might not be quickly internalized, but rather presents antigens to the T cells, leading to a range of significant immunological responses.