Femtosecond protein nanocrystallography-data analysis methods

• Published in: Optics express Vol. 18; no. 6; pp. 5713 - 5723
• Main Authors: Kirian, Richard A, Wang, Xiaoyu, Weierstall, Uwe, Schmidt, Kevin E, Spence, John C H, Hunter, Mark, Fromme, Petra, White, Thomas, Chapman, Henry N, Holton, James
• Format: Journal Article
• Language: English
• Published: United States 15.03.2010
• Subjects: Algorithms; Pattern Recognition, Automated - methods; Photosystem I Protein Complex - ultrastructure; X-Ray Diffraction - methods

X-ray diffraction patterns may be obtained from individual submicron protein nanocrystals using a femtosecond pulse from a free-electron X-ray laser. Many "single-shot" patterns are read out every second from a stream of nanocrystals lying in random orientations. The short pulse terminates...