Factors affecting the genetic engineering of plants by microprojectile bombardment

• Published in: Biotechnology advances Vol. 13; no. 4; pp. 631 - 651
• Main Authors: Southgate, E.M., Davey, M.R., Power, J.B., Marchant, R.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Inc 1995; New York, NY Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biolistics; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; gene delivery; Genetic engineering; Genetic engineering applications; Genetic technics; Genetics and breeding of economic plants; Methods. Procedures. Technologies; Microprojectile; particle bombardment; particle gun; Plant breeding: fundamental aspects and methodology; plant transformation; Transgenic animals and transgenic plants; Transgenic plants; particle bombardment; gene delivery; Biolistics; Microprojectile; particle gun; plant transformation; Microprojectile bombardment; Monocotyledones; Angiospermae; Spermatophyta; Transgenic plant; Review; Method; Cultivated plant; Genetic transfer
• ISSN: 0734-9750; 1873-1899
• DOI: 10.1016/0734-9750(95)02008-X

Since its development in the mid-1980s, microprojectile bombardment has been widely employed as a method for direct gene transfer into a wide range of plants, including the previously difficult-to-transform monocotyledonous species. Although the numerous instruments available for microprojectile-mediated gene delivery and their applications have been widely discussed, less attention has been paid to the critical factors which affect the efficiency of this method of gene delivery. In this review we do not wish to describe the array of devices used for microprojectile delivery or their uses which have already been definitively described, but instead wish to report on research developments investigating the factors which affect microprojectile-mediated transformation of plants.