Proteomic analysis of the cellular proteins induced by adaptive concentrations of hydrogen peroxide in human U937 cells
When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this...
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Published in | Experimental & molecular medicine Vol. 34; no. 5; pp. 374 - 378 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
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Springer Nature B.V
30.11.2002
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Abstract | When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 micromM H(2)O(2) for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H(2)O(2) treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H(2)O(2)-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions. |
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AbstractList | When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 µM H2O2 for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H2O2 treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H2O2-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions. When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 micromM H(2)O(2) for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H(2)O(2) treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H(2)O(2)-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions. When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 mu M H2O2 for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H2O2 treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H2O2-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions. |
Author | Oh, Seung Hyun Um, Hong-Duck Kim, Do Kyun Kim, Kil Soo Lee, Seung-Sook Choi, Kun Ho Seong, Je Kyung |
Author_xml | – sequence: 1 givenname: Je Kyung surname: Seong fullname: Seong, Je Kyung organization: Laboratory of Developmental Biology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea – sequence: 2 givenname: Do Kyun surname: Kim fullname: Kim, Do Kyun – sequence: 3 givenname: Kun Ho surname: Choi fullname: Choi, Kun Ho – sequence: 4 givenname: Seung Hyun surname: Oh fullname: Oh, Seung Hyun – sequence: 5 givenname: Kil Soo surname: Kim fullname: Kim, Kil Soo – sequence: 6 givenname: Seung-Sook surname: Lee fullname: Lee, Seung-Sook – sequence: 7 givenname: Hong-Duck surname: Um fullname: Um, Hong-Duck |
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SubjectTerms | Adaptation, Physiological - drug effects Adaptation, Physiological - physiology Cells, Cultured Humans Hydrogen Peroxide - pharmacology Proteins - drug effects Proteins - metabolism Proteomics - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization U937 Cells |
Title | Proteomic analysis of the cellular proteins induced by adaptive concentrations of hydrogen peroxide in human U937 cells |
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