Thiolation of Myco-Synthesized Fe3O4-NPs: A Novel Promising Tool for Penicillium expansium Laccase Immobilization to Decolorize Textile Dyes and as an Application for Anticancer Agent

Environmental pollution due to the continuous uncontrolled discharge of toxic dyes into the water bodies provides insight into the need to eliminate pollutants prior to discharge is significantly needed. Recently, the combination of conventional chemotherapeutic agents and nanoparticles has attracte...

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Published inJournal of fungi (Basel) Vol. 8; no. 1; p. 71
Main Authors El-Shora, Hamed M., Khateb, Aiah M., Darwish, Doaa B., El-Sharkawy, Reyad M.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.01.2022
MDPI
Subjects
Acids
Adsorption
Antitumor agents
biocatalyst
Biodegradation
Bioremediation
Biosynthesis
Cancer
Carboplatin
Cell lines
Cellulose
Chemicals
Chemotherapy
Chromatography
Cytotoxicity
Decolorization
Dyes
Enzymes
Fourier transforms
Immobilization
immobilized laccase
Iron oxides
Laccase
Nanocomposites
Nanoparticles
Penicillium expansium
Pollutants
thiolated nanosupport
Wastewater
United States > US
Cairo Egypt
Egypt
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Abstract Environmental pollution due to the continuous uncontrolled discharge of toxic dyes into the water bodies provides insight into the need to eliminate pollutants prior to discharge is significantly needed. Recently, the combination of conventional chemotherapeutic agents and nanoparticles has attracted considerable attention. Herein, the magnetic nanoparticles (Fe3O4-NPs) were synthesized using metabolites of Aspergillus niger. Further, the surfaces of Fe3O4-NPs were functionalized using 3-mercaptoproionic acid as confirmed by XRD, TEM, and SEM analyses. A purified P. expansum laccase was immobilized onto Fe3O4/3-MPA-SH and then the developed immobilized laccase (Fe3O4/3-MPA-S-S-laccase) was applied to achieve redox-mediated degradation of different dyes. The Fe3O4/3-MPA-S-S-laccase exhibited notably improved stability toward pH, temperature, organic solvents, and storage periods. The Fe3O4/3-MPA-S-S-laccase exhibited appropriate operational stability while retaining 84.34% of its initial activity after 10 cycles. The catalytic affinity (Kcat/Km) of the immobilized biocatalyst was increased above 10-fold. The experimental data showed remarkable improvement in the dyes’ decolorization using the immobilized biocatalyst in the presence of a redox mediator in seven successive cycles. Thus, the prepared novel nanocomposite-laccase can be applied as an alternative promising strategy for bioremediation of textile wastewater. The cytotoxic level of carboplatin and Fe3O4-NPs singly or in combination on various cell lines was concentration-dependent.
AbstractList Environmental pollution due to the continuous uncontrolled discharge of toxic dyes into the water bodies provides insight into the need to eliminate pollutants prior to discharge is significantly needed. Recently, the combination of conventional chemotherapeutic agents and nanoparticles has attracted considerable attention. Herein, the magnetic nanoparticles (Fe3O4-NPs) were synthesized using metabolites of Aspergillus niger. Further, the surfaces of Fe3O4-NPs were functionalized using 3-mercaptoproionic acid as confirmed by XRD, TEM, and SEM analyses. A purified P. expansum laccase was immobilized onto Fe3O4/3-MPA-SH and then the developed immobilized laccase (Fe3O4/3-MPA-S-S-laccase) was applied to achieve redox-mediated degradation of different dyes. The Fe3O4/3-MPA-S-S-laccase exhibited notably improved stability toward pH, temperature, organic solvents, and storage periods. The Fe3O4/3-MPA-S-S-laccase exhibited appropriate operational stability while retaining 84.34% of its initial activity after 10 cycles. The catalytic affinity (Kcat/Km) of the immobilized biocatalyst was increased above 10-fold. The experimental data showed remarkable improvement in the dyes’ decolorization using the immobilized biocatalyst in the presence of a redox mediator in seven successive cycles. Thus, the prepared novel nanocomposite-laccase can be applied as an alternative promising strategy for bioremediation of textile wastewater. The cytotoxic level of carboplatin and Fe3O4-NPs singly or in combination on various cell lines was concentration-dependent.
Environmental pollution due to the continuous uncontrolled discharge of toxic dyes into the water bodies provides insight into the need to eliminate pollutants prior to discharge is significantly needed. Recently, the combination of conventional chemotherapeutic agents and nanoparticles has attracted considerable attention. Herein, the magnetic nanoparticles (Fe 3 O 4 -NPs) were synthesized using metabolites of Aspergillus niger . Further, the surfaces of Fe 3 O 4 -NPs were functionalized using 3-mercaptoproionic acid as confirmed by XRD, TEM, and SEM analyses. A purified P. expansum laccase was immobilized onto Fe 3 O 4 /3-MPA-SH and then the developed immobilized laccase (Fe 3 O 4 /3-MPA-S-S-laccase) was applied to achieve redox-mediated degradation of different dyes. The Fe 3 O 4 /3-MPA-S-S-laccase exhibited notably improved stability toward pH, temperature, organic solvents, and storage periods. The Fe 3 O 4 /3-MPA-S-S-laccase exhibited appropriate operational stability while retaining 84.34% of its initial activity after 10 cycles. The catalytic affinity (K cat /K m ) of the immobilized biocatalyst was increased above 10-fold. The experimental data showed remarkable improvement in the dyes’ decolorization using the immobilized biocatalyst in the presence of a redox mediator in seven successive cycles. Thus, the prepared novel nanocomposite-laccase can be applied as an alternative promising strategy for bioremediation of textile wastewater. The cytotoxic level of carboplatin and Fe 3 O 4 -NPs singly or in combination on various cell lines was concentration-dependent.
Author El-Shora, Hamed M
Khateb, Aiah M
Darwish, Doaa B
El-Sharkawy, Reyad M
AuthorAffiliation 1 Department of Botany, Faculty of Science, Mansoura University, Mansoura 35511, Egypt; d_darwish@mans.edu.eg
3 Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt; r.m.elsharkawy@fsc.bu.edu.eg
2 Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Medina 41411, Saudi Arabia; akhateb@taibahu.edu.sa
AuthorAffiliation_xml – name: 1 Department of Botany, Faculty of Science, Mansoura University, Mansoura 35511, Egypt; d_darwish@mans.edu.eg
– name: 2 Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Medina 41411, Saudi Arabia; akhateb@taibahu.edu.sa
– name: 3 Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt; r.m.elsharkawy@fsc.bu.edu.eg
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SSID ssj0001586993
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Snippet Environmental pollution due to the continuous uncontrolled discharge of toxic dyes into the water bodies provides insight into the need to eliminate pollutants...
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StartPage 71
SubjectTerms Acids
Adsorption
Antitumor agents
biocatalyst
Biodegradation
Bioremediation
Biosynthesis
Cancer
Carboplatin
Cell lines
Cellulose
Chemicals
Chemotherapy
Chromatography
Cytotoxicity
Decolorization
Dyes
Enzymes
Fourier transforms
Immobilization
immobilized laccase
Iron oxides
Laccase
Nanocomposites
Nanoparticles
Penicillium expansium
Pollutants
thiolated nanosupport
Wastewater
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Title Thiolation of Myco-Synthesized Fe3O4-NPs: A Novel Promising Tool for Penicillium expansium Laccase Immobilization to Decolorize Textile Dyes and as an Application for Anticancer Agent
URI https://www.proquest.com/docview/2621318591/abstract/
https://search.proquest.com/docview/2621659135
https://pubmed.ncbi.nlm.nih.gov/PMC8777717
https://doaj.org/article/9e522c94d9f84775ae7c2df71b405951
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