The Use of Non-Uniform Deuterium Labelling [‘NMR-Window’] To Study the NMR Structure of a 21mer RNA Hairpin

The first synthesis of a non-uniformly deuterium labelled 21mer RNA is reported using our ‘NMR-window’ concept, showing its unique application in the unambiguous NMR assignment of the non-exchangeable aromatic and sugar protons.

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Bibliographic Details
Published inNucleic acids research Vol. 24; no. 7; pp. 1187 - 1194
Main Authors Földesi, A., Yamakage, S.-I., Nilsson, F. P. R., Maltseva, T. V., Chattopadhyaya, J.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.04.1996
Subjects
Deuterium
Hydrogen Bonding
Magnetic Resonance Spectroscopy - methods
Nucleic Acid Conformation
Oligoribonucleotides - chemistry
POSITION
BASES
ACID
PROTECTION
PHOSPHORAMIDITES
DNA
ESCHERICHIA-COLI
H-1-NMR WINDOW
H-1
CHEMICAL SYNTHESIS
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Summary:The first synthesis of a non-uniformly deuterium labelled 21mer RNA is reported using our ‘NMR-window’ concept, showing its unique application in the unambiguous NMR assignment of the non-exchangeable aromatic and sugar protons.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/24.7.1187