Culture, immortalization, and characterization of human meibomian gland epithelial cells

• Published in: Investigative ophthalmology & visual science Vol. 51; no. 8; pp. 3993 - 4005
• Main Authors: Liu, Shaohui, Hatton, Mark P, Khandelwal, Payal, Sullivan, David A
• Format: Journal Article
• Language: English
• Published: United States Association for Research in Vision and Ophthalmology, Inc 01.08.2010
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, Polyomavirus Transforming - genetics; Antigens, Polyomavirus Transforming - metabolism; Cell Culture Techniques; Cell Division; Cell Line; Cell Proliferation; Epithelial Cells - cytology; Epithelial Cells - metabolism; Female; Fluorescent Antibody Technique, Indirect; Genetic Vectors; Humans; Karyotyping; Lipid Metabolism; Male; Meibomian Glands - cytology; Meibomian Glands - metabolism; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; Telomerase - genetics; Telomerase - metabolism

Meibomian gland epithelial cells are essential in maintaining the health and integrity of the ocular surface. However, very little is known about their physiological regulation. In this study, the cellular control mechanisms were explored, first to establish a defined culture system for the maintena...