A Multistep Workflow to Evaluate Newly Generated iPSCs and Their Ability to Generate Different Cell Types

• Published in: Methods and protocols Vol. 4; no. 3; p. 50
• Main Authors: Chen, Carol X.-Q., Abdian, Narges, Maussion, Gilles, Thomas, Rhalena A., Demirova, Iveta, Cai, Eddie, Tabatabaei, Mahdieh, Beitel, Lenore K., Karamchandani, Jason, Fon, Edward A., Durcan, Thomas M.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 19.07.2021; MDPI
• Subjects: Alzheimer's disease; Cell differentiation; cortical neurons; Fibroblasts; Gene expression; Genomes; genomic integrity; Genomics; human-induced pluripotent stem cells; Inhibitory postsynaptic potentials; Morphology; neural progenitor cells; Neurogenesis; Pluripotency; quality control; Somatic cells; Stem cells; trilineage differentiation; Canada; United States > US

Induced pluripotent stem cells (iPSCs) derived from human somatic cells have created new opportunities to generate disease-relevant cells. Thus, as the use of patient-derived stem cells has become more widespread, having a workflow to monitor each line is critical. This ensures iPSCs pass a suite of...