Detection of 123 bp fragment of insertion element IS6110 Mycobacterium tuberculosis for diagnosis of extrapulmonary tuberculosis

• Published in: Indian journal of medical microbiology Vol. 30; no. 2; pp. 182 - 186
• Main Authors: Maurya, AK, Kant, S, Nag, VL, Kushwaha, RAS, Dhole, TN
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.04.2012; Elsevier Limited
• Subjects: Adult; Bacilli; Clinical isolates; Clinical Laboratory Techniques - methods; DNA Primers - genetics; DNA Transposable Elements; DNA, Bacterial - genetics; Extrapulmonary tuberculosis; Female; Hospitals; Humans; India - epidemiology; Industrialized nations; IS6110; Lymph nodes; Male; Microbiology; Molecular Diagnostic Techniques - methods; Mycobacterium tuberculosis; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - growth & development; Mycobacterium tuberculosis - isolation & purification; Mycobacterium tuberculosis complex; Polymerase chain reaction; Polymerase Chain Reaction - methods; Prevalence; Primers; Sensitivity and Specificity; Synovial fluid; Tuberculosis; Tuberculosis - diagnosis; Tuberculosis - epidemiology; India; Extrapulmonary tuberculosis; IS6110; Mycobacterium tuberculosis complex; polymerase chain reaction

Purpose: Extrapulmonary tuberculosis (EPTB) is emerging problem in developing and developed countries. The diagnosis of EPTB in its different clinical presentations remains a true challenge. IS6110-based polymerase chain reaction (PCR) is used for rapid identification and positivity rate of the Myco...