Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2 - Miranda, Jaclyn A.; Steward, Grieg F. (Journal of virological methods, 2017)
Keywords: bacteriophages; cDNA synthesis; Data Accuracy; DNA Primers; DNA, Complementary - genetics; enzymes; Levivirus - genetics; quantitative polymerase chain reaction; Reproducibility of Results; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - standards; Reverse Transcription; RNA; RNA, Viral - genetics; RNA, Viral - metabolism; RT-qPCR; Sensitivity and Specificity; Virus quantification; RT; RNA; rRNA; s.d; mRNA; cDNA; AMV; Virus quantification; RT-qPCR; Cq; MMLV; cDNA synthesis