First description of betalains biosynthesis in an aquatic organism: characterization of 4,5‐DOPA‐extradiol‐dioxygenase activity in the cyanobacteria Anabaena cylindrica

• Published in: Microbial biotechnology Vol. 13; no. 6; pp. 1948 - 1959
• Main Authors: Guerrero‐Rubio, María Alejandra, García‐Carmona, Francisco, Gandía‐Herrero, Fernando
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.11.2020; John Wiley and Sons Inc; Wiley
• Subjects: Anabaena; Anabaena cylindrica; Aquatic Organisms; Ascorbic acid; Betalains; Biosynthesis; Culture media; Cyanobacteria; Dihydroxyphenylalanine; Dioxygenase; Dioxygenases - genetics; Enzymes; Ethylenediaminetetraacetic acids; Fungi; Genomes; Gluconacetobacter; Levodopa; Microorganisms; Nitrogen; Phylogenetics; Phylogeny; Pigments; Prokaryotes; Searching; Sodium

This paper describes molecular and functional characterization of a novel dioxygenase enzyme from the aquatic cyanobacterium Anabaena cylindrica. The enzyme showed a strong inhibition by excess of the precursor L‐DOPA. However, its heterologous expression has allowed detecting the formation of the m...