Lysine-Specific Demethylase 4A Regulates Osteogenic Differentiation via Regulating the Binding Ability of H3K9me3 with the Promoters of Runx2, Osterix and Osteocalcin

• Published in: Journal of biomedical nanotechnology Vol. 16; no. 6; p. 899
• Main Authors: Qin, Guozhong, Li, Yikai, Wang, Haibin, Yang, Junxing, Chen, Qunqun, Tang, Hongyu, Wang, Yueqi, Zhang, Meng, Jiang, Tao, Lin, Songqing, Huo, Shaochuan
• Format: Journal Article
• Language: English
• Published: United States 01.06.2020
• Subjects: Animals; Cell Differentiation; Core Binding Factor Alpha 1 Subunit - genetics; Histone Demethylases - physiology; Lysine; Osteoblasts - metabolism; Osteocalcin - genetics; Osteogenesis - genetics; Rats; Rats, Sprague-Dawley; Receptors, Oxytocin; Transcription Factors - genetics; Transcription Factors - metabolism
• DOI: 10.1166/jbn.2020.2929
• ISSN: 1550-7033

A well-studied subject of epigenetics, the histone methylation located at lysine and arginine is overseen via methyltransferases and demethylases. Lysine-specific demethylase 4A (KDM4A) comprises a lysine demethylase and possesses specificity for H3K9me3 and H3K36me3, which is capable of being used in order to activate histone transcription. Our team examined the expression of KDM4A within Sprague Dawley (SD) rats and further investigated the mechanism via which this phenomena regulates osteogenic variation within the present study. The overexpression of KDM4A facilitated the process of osteoblast differentiation in bone mesenchymal stem cells (BMSC), while the knocking down differentiation via osteoblast was restrained via the suppression of the expression of Runx2, Osterix, alkaline phosphatase (ALP), and osteocalcin (OCN). Knocking down KDM4A lowered levels of the promoter expression of Runx2, osterix, and OCN, and raised levels of H3K27me3 expression. The results demonstrated that KDM4A possesses a crucial role within the differentiation of osteoblasts and furthermore regulates the expression of Runx2, Osterix, and OCN via H3K9me3. The present research may provide new insights into the treatment of bone healing.