Whole blood and leukocyte RNA isolation for gene expression analyses

• Published in: Physiological genomics Vol. 19; no. 3; pp. 247 - 254
• Main Authors: Feezor, Robert J, Baker, Henry V, Mindrinos, Michael, Hayden, Doug, Tannahill, Cynthia L, Brownstein, Bernard H, Fay, Adrian, MacMillan, Sandra, Laramie, Jason, Xiao, Wenzhong, Moldawer, Lyle L, Cobb, J. Perren, Laudanski, Krzysztof, Miller-Graziano, Carol L, Maier, Ronald V, Schoenfeld, David, Davis, Ronald W, Tompkins, Ronald G, Inflammation and Host Response to Injury, Large-Scale Collaborative Research Program
• Format: Journal Article
• Language: English
• Published: United States Am Physiological Soc 17.11.2004
• Subjects: Adolescent; Adult; Aged; Antigens, Bacterial - metabolism; Blood Specimen Collection - standards; Cluster Analysis; Enterotoxins - metabolism; Female; Gene Expression Profiling - methods; Gene Expression Profiling - standards; Gene Expression Profiling - statistics & numerical data; Humans; Leukocytes - chemistry; Male; Middle Aged; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Array Sequence Analysis - standards; Oligonucleotide Array Sequence Analysis - statistics & numerical data; Reproducibility of Results; Research Design - standards; RNA - blood; RNA - genetics; Specimen Handling - standards; Staphylococcus; Wounds and Injuries - blood

1 Department of Surgery, University of Florida College of Medicine, Gainesville, Florida 2 Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 3 Stanford Genome Technology Center, Palo Alto, California 4 Department of Surgery, Shriners B...