A human microprotein that interacts with the mRNA decapping complex

• Published in: Nature chemical biology Vol. 13; no. 2; pp. 174 - 180
• Main Authors: D'Lima, Nadia G, Ma, Jiao, Winkler, Lauren, Chu, Qian, Loh, Ken H, Corpuz, Elizabeth O, Budnik, Bogdan A, Lykke-Andersen, Jens, Saghatelian, Alan, Slavoff, Sarah A
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.02.2017; Nature Publishing Group
• Subjects: 14; 14/63; 631/1647/296; 631/337/1645; 631/337/384; 631/92/611; 82; 82/58; Animals; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Carrier Proteins - metabolism; Cell Biology; Cells, Cultured; Cellular biology; Cercopithecus aethiops; Chemistry; Chemistry/Food Science; COS Cells; Decay; Endoribonucleases - chemistry; Endoribonucleases - metabolism; Humans; Polypeptides; Proteins; RNA Caps - metabolism; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-protein interactions

Mass-spectrometry-based proteomics led to the identification of NoBody, a microprotein translated from LINC01420 RNA, which interacts with enhancer of decapping 4 (EDC4) and negatively regulates 5′-to-3′ mRNA decay. Proteomic detection of non-annotated microproteins indicates the translation of hund...