Proteomic Identification of Target Proteins of Thiodigalactoside in White Adipose Tissue from Diet-Induced Obese Rats

• Published in: International journal of molecular sciences Vol. 16; no. 7; pp. 14441 - 14463
• Main Authors: Parray, Hilal, Yun, Jong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.06.2015; MDPI
• Subjects: Adipose Tissue, White - drug effects; Adipose Tissue, White - metabolism; Animals; Annexin A2 - genetics; Annexin A2 - metabolism; Body fat; Carbonic Anhydrases - genetics; Carbonic Anhydrases - metabolism; Diet, High-Fat - adverse effects; Galectin 1 - antagonists & inhibitors; Galectin 1 - metabolism; Isoenzymes - genetics; Isoenzymes - metabolism; L-Lactate Dehydrogenase - genetics; L-Lactate Dehydrogenase - metabolism; Obesity; Obesity - etiology; Obesity - genetics; Obesity - metabolism; Phosphatidylethanolamine Binding Protein - genetics; Phosphatidylethanolamine Binding Protein - metabolism; Proteome - drug effects; Proteome - genetics; Proteome - metabolism; Proteomics; Rats; Rats, Sprague-Dawley; Rodents; Thiogalactosides - pharmacology; Tissues; Voltage-Dependent Anion Channel 1 - genetics; Voltage-Dependent Anion Channel 1 - metabolism; thiodigalactoside; white adipose tissue; obesity; galectin; proteome

Previously, galectin-1 (GAL1) was found to be up-regulated in obesity-prone subjects, suggesting that use of a GAL1 inhibitor could be a novel therapeutic approach for treatment of obesity. We evaluated thiodigalactoside (TDG) as a potent inhibitor of GAL1 and identified target proteins of TDG by pe...