Regeneration of Periodontal Ligament for Apatite-coated Tooth-shaped Titanium Implants with and without Occlusion Using Rat Molar Model

Regeneration of periodontal ligament (PDL) is still a major issue in implant dentistry. In this study, we evaluated the effects of hydroxyapatite (HA) coating and occlusion on the regeneration of PDL around tooth-shaped titanium implants with root form. Three groups of tooth-shaped titanium implants...

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Published inJournal of Hard Tissue Biology Vol. 21; no. 2; pp. 189 - 202
Main Authors Kano, Takamitsu, Yamamoto, Ryuji, Miyashita, Akira, Komatsu, Koichiro, Hayakawa, Tohru, Sato, Mitsunobu, Oida, Shinichiro
Format Journal Article
LanguageEnglish
Published THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY 2012
Subjects
Alkaline phosphatase
Bone (alveolar)
Bone implants
Coatings
Collagen
Dental implant
Dental roots
Dentistry
Fibers
Hydroxyapatite
Hydroxyapatite coating
Molars
Occlusion
Osseointegration
periodontal ligament
Periodontium
Regeneration
Teeth
Titanium
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Abstract Regeneration of periodontal ligament (PDL) is still a major issue in implant dentistry. In this study, we evaluated the effects of hydroxyapatite (HA) coating and occlusion on the regeneration of PDL around tooth-shaped titanium implants with root form. Three groups of tooth-shaped titanium implants, namely, non-HA-coated without occlusion (HA-/OCL-), HA-coated without occlusion (HA+/OCL-), and HA-coated with occlusion (HA+/OCL+), were immediately implanted into extracted tooth sockets with remaining PDL of rat molar model and the regeneration of PDL was examined histomorphometrically and histologically. Direct bony attachments were formed at the HA+/OCL- implant interface. In contrast, soft peri-implant tissue was distinctly seen between the HA+/OCL+ implant surface and surrounding alveolar bones. The shape of the load-time curve for the HA+/OCL+ group was similar to that for natural teeth. Peri-implant tissue of the HA+/OCL+ implants exhibited alkaline phosphatase with a distribution and activity similar to those of the PDL. Collagen fiber bundles were found functionally oriented in some regions of the peri-implant tissue. These findings indicate that the remaining PDL tissue around extracted sockets has the ability to regenerate bone and PDL-like tissues on HA-coated tooth-shaped implants. Occlusal loads to the HA-coated implants may induce regeneration of PDL-like tissue in the peri-implant tissue.
AbstractList Regeneration of periodontal ligament (PDL) is still a major issue in implant dentistry. In this study, we evaluated the effects of hydroxyapatite (HA) coating and occlusion on the regeneration of PDL around tooth-shaped titanium implants with root form. Three groups of tooth-shaped titanium implants, namely, non-HA-coated without occlusion (HA-/OCL-), HA-coated without occlusion (HA+/OCL-), and HA-coated with occlusion (HA+/OCL+), were immediately implanted into extracted tooth sockets with remaining PDL of rat molar model and the regeneration of PDL was examined histomorphometrically and histologically. Direct bony attachments were formed at the HA+/OCL- implant interface. In contrast, soft peri-implant tissue was distinctly seen between the HA+/OCL+ implant surface and surrounding alveolar bones. The shape of the load-time curve for the HA+/OCL+ group was similar to that for natural teeth. Peri-implant tissue of the HA+/OCL+ implants exhibited alkaline phosphatase with a distribution and activity similar to those of the PDL. Collagen fiber bundles were found functionally oriented in some regions of the peri-implant tissue. These findings indicate that the remaining PDL tissue around extracted sockets has the ability to regenerate bone and PDL-like tissues on HA-coated tooth-shaped implants. Occlusal loads to the HA-coated implants may induce regeneration of PDL-like tissue in the peri-implant tissue.
Author Yamamoto, Ryuji
Hayakawa, Tohru
Oida, Shinichiro
Sato, Mitsunobu
Kano, Takamitsu
Miyashita, Akira
Komatsu, Koichiro
Author_xml – sequence: 1
  fullname: Kano, Takamitsu
  organization: Department of Fixed Prosthodontics, Tsurumi University School of Dental Medicine
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  fullname: Yamamoto, Ryuji
  organization: Department of Biochemistry and Molecular Biology,Tsurumi University School of Dental Medicine
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  fullname: Miyashita, Akira
  organization: Department of Fixed Prosthodontics, Tsurumi University School of Dental Medicine
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  fullname: Komatsu, Koichiro
  organization: Department of Pharmacology,Tsurumi University School of Dental Medicine
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  fullname: Hayakawa, Tohru
  organization: Department of Dental Engineering,Tsurumi University School of Dental Medicine
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  fullname: Sato, Mitsunobu
  organization: Coordination Engineering Laboratory, Faculty of Engineering, Kogakuin University
– sequence: 7
  fullname: Oida, Shinichiro
  organization: Department of Biochemistry and Molecular Biology,Tsurumi University School of Dental Medicine
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SubjectTerms Alkaline phosphatase
Bone (alveolar)
Bone implants
Coatings
Collagen
Dental implant
Dental roots
Dentistry
Fibers
Hydroxyapatite
Hydroxyapatite coating
Molars
Occlusion
Osseointegration
periodontal ligament
Periodontium
Regeneration
Teeth
Titanium
Title Regeneration of Periodontal Ligament for Apatite-coated Tooth-shaped Titanium Implants with and without Occlusion Using Rat Molar Model
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