Protective effects of salidroside on hypoxia/reoxygenation injury by sodium hydrosulfite in PC12 cells

• Published in: Pharmaceutical biology Vol. 45; no. 8; pp. 604 - 612
• Main Authors: Chen, Z, Lu, Y, Wang, Z, Tao, X, Wei, D
• Format: Journal Article
• Language: English
• Published: Informa UK Ltd 01.01.2007; Taylor & Francis
• Subjects: cell death; cell lines; culture media; cultured cells; cytotoxicity; dosage; dose response; herbal medicines; Hypoxia; hypoxia-reoxygenation injury; medicinal plants; medicinal properties; neoplasms; Oriental traditional medicine; pathophysiology; PC12 cells, salidrosid; protective effect; reoxygenation, Na; Rhodiola rosea; salidroside; sodium hydrosulfite; viability
• ISSN: 1388-0209; 1744-5116
• DOI: 10.1080/13880200701538666

Abstract Hypoxia reoxygenation causes cellular injury and death associated with many pathophysiologic conditions, including respiratory disorders, myocardial ischemia, and tumour progression diseases. Neuronal pheochromocytoma (PC12) cells are widely used as a model system for neurologic research and are subject to chemical hypoxia induced with sodium hydrosulfite (Na2S2O4), a common oxygen-consuming agent. Salidroside, which is the main active component of the famous traditional Chinese herb Rhodiola rosea. L. (Crassulaceae), has been proved to possess many bioactivities. In this article, we studied the protective effects of salidroside on hypoxia reoxygenation injury in PC12 cells induced by Na2S2O4. Cultures of PC12 cells were exposed for 1 h to 10 mM Na2S2O4 for hypoxia, followed by reoxygenation for 2 h. The results showed that salidroside was very stable in medium and was not harmful to PC12 cells at the experimental concentrations of 0 ∼ 200 µg mL. The cytoprotection by salidroside was dose-dependent, and the cell viability was 41.8 ± 5.7%, 62.4 ± 4.1%, and 92.2 ± 3.7% at 0, 50, and 100 µg mL of salidroside, respectively. The level of released LDH significantly decreased from 513.5 ± 5.5% (without salidroside) to 258.1 ± 6.3% (with 100 µg mL salidroside). Flow cytometry was performed to measure apoptotic rate. The results of flow cytometry assay indicated that the apoptotic rate was 17.0 ± 1.2% after hypoxia reoxygenation injury. When the cells were treated with salidroside 12.5, 50 and 100 µg mL, the apoptotic rate was 9.5 ± 0.9%, 7.4 ± 0.5%, and 4.5 ± 0.4%, respectively. In addition, our results were confirmed by inspection of cell morphology of PC12 cells. Treatment with salidroside (12.5, 50, 100 µg mL) significantly prevented the cells from morphologic changes. All the above results showed salidroside could effectively protect PC12 cell against hypoxia reoxygenation injury.