Characterization of all human male synaptonemal complexes by subtelomere multiplex-FISH
During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous chromosomes synapse, a proteinaceous structure called synaptonemal complex (SC) appears along the pairing axis and meiotic recombination takes plac...
Saved in:
| Published in | Cytogenetic and genome research Vol. 107; no. 1-2; pp. 18 - 21 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Basel, Switzerland
S. Karger AG
01.01.2004
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous chromosomes synapse, a proteinaceous structure called synaptonemal complex (SC) appears along the pairing axis and meiotic recombination takes place. The existence of immunolabeling techniques for SC proteins (SCP1, SCP2 and SCP3) and for DNA mismatch repair proteins present in late recombination nodules (MLH1) allow analyses of both synapsis and meiotic recombination in the gametocyte I. In situ hybridization methods can be applied afterwards because chromatin is preserved during cell fixation for immunoanalysis. The combination of both methodologies allows the analysis of synapsis and the creation of recombination maps for each bivalent. In this work we apply the seven-fluorochrome subtelomere-specific multiplex FISH assay (stM-FISH) to human male meiotic cells previously labeled by immunofluorescence (SCP1, SCP3, MLH1, CENP) to assess its utility for human SC karyotyping. This FISH method consists of microdissected subtelomeric probes labeled combinatorially with seven different fluorochromes. Results prove its usefulness for the identification of all human SCs. Furthermore, by labeling subtelomeric regions this one-single-step method enables the characterization of interstitial and terminal SC fragments and SC delineation even if superposition is present in pachytene spreads. |
|---|---|
| AbstractList | During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous chromosomes synapse, a proteinaceous structure called synaptonemal complex (SC) appears along the pairing axis and meiotic recombination takes place. The existence of immunolabeling techniques for SC proteins (SCP1, SCP2 and SCP3) and for DNA mismatch repair proteins present in late recombination nodules (MLH1) allow analyses of both synapsis and meiotic recombination in the gametocyte I. In situ hybridization methods can be applied afterwards because chromatin is preserved during cell fixation for immunoanalysis. The combination of both methodologies allows the analysis of synapsis and the creation of recombination maps for each bivalent. In this work we apply the seven-fluorochrome subtelomere-specific multiplex FISH assay (stM-FISH) to human male meiotic cells previously labeled by immunofluorescence (SCP1, SCP3, MLH1, CENP) to assess its utility for human SC karyotyping. This FISH method consists of microdissected subtelomeric probes labeled combinatorially with seven different fluorochromes. Results prove its usefulness for the identification of all human SCs. Furthermore, by labeling subtelomeric regions this one-single-step method enables the characterization of interstitial and terminal SC fragments and SC delineation even if superposition is present in pachytene spreads. Copyright © 2004 S. Karger AG, Basel During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous chromosomes synapse, a proteinaceous structure called synaptonemal complex (SC) appears along the pairing axis and meiotic recombination takes place. The existence of immunolabeling techniques for SC proteins (SCP1, SCP2 and SCP3) and for DNA mismatch repair proteins present in late recombination nodules (MLH1) allow analyses of both synapsis and meiotic recombination in the gametocyte I. In situ hybridization methods can be applied afterwards because chromatin is preserved during cell fixation for immunoanalysis. The combination of both methodologies allows the analysis of synapsis and the creation of recombination maps for each bivalent. In this work we apply the seven-fluorochrome subtelomere-specific multiplex FISH assay (stM-FISH) to human male meiotic cells previously labeled by immunofluorescence (SCP1, SCP3, MLH1, CENP) to assess its utility for human SC karyotyping. This FISH method consists of microdissected subtelomeric probes labeled combinatorially with seven different fluorochromes. Results prove its usefulness for the identification of all human SCs. Furthermore, by labeling subtelomeric regions this one-single-step method enables the characterization of interstitial and terminal SC fragments and SC delineation even if superposition is present in pachytene spreads. During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous chromosomes synapse, a proteinaceous structure called synaptonemal complex (SC) appears along the pairing axis and meiotic recombination takes place. The existence of immunolabeling techniques for SC proteins (SCP1, SCP2 and SCP3) and for DNA mismatch repair proteins present in late recombination nodules (MLH1) allow analyses of both synapsis and meiotic recombination in the gametocyte I. In situ hybridization methods can be applied afterwards because chromatin is preserved during cell fixation for immunoanalysis. The combination of both methodologies allows the analysis of synapsis and the creation of recombination maps for each bivalent. In this work we apply the seven-fluorochrome subtelomere-specific multiplex FISH assay (stM-FISH) to human male meiotic cells previously labeled by immunofluorescence (SCP1, SCP3, MLH1, CENP) to assess its utility for human SC karyotyping. This FISH method consists of microdissected subtelomeric probes labeled combinatorially with seven different fluorochromes. Results prove its usefulness for the identification of all human SCs. Furthermore, by labeling subtelomeric regions this one-single-step method enables the characterization of interstitial and terminal SC fragments and SC delineation even if superposition is present in pachytene spreads. Copyright [copy 2004 S. Karger AG, Basel During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous chromosomes synapse, a proteinaceous structure called synaptonemal complex (SC) appears along the pairing axis and meiotic recombination takes place. The existence of immunolabeling techniques for SC proteins (SCP1, SCP2 and SCP3) and for DNA mismatch repair proteins present in late recombination nodules (MLH1) allow analyses of both synapsis and meiotic recombination in the gametocyte I. In situ hybridization methods can be applied afterwards because chromatin is preserved during cell fixation for immunoanalysis. The combination of both methodologies allows the analysis of synapsis and the creation of recombination maps for each bivalent. In this work we apply the seven-fluorochrome subtelomere-specific multiplex FISH assay (stM-FISH) to human male meiotic cells previously labeled by immunofluorescence (SCP1, SCP3, MLH1, CENP) to assess its utility for human SC karyotyping. This FISH method consists of microdissected subtelomeric probes labeled combinatorially with seven different fluorochromes. Results prove its usefulness for the identification of all human SCs. Furthermore, by labeling subtelomeric regions this one-single-step method enables the characterization of interstitial and terminal SC fragments and SC delineation even if superposition is present in pachytene spreads. |
| Author | Egozcue, J. Oliver-Bonet, M. Codina-Pascual, M. Navarro, J. Murcia, V. Benet, J. Sarquella, J. Kraus, J. Speicher, M.R. |
| Author_xml | – sequence: 1 givenname: M. surname: Codina-Pascual fullname: Codina-Pascual, M. – sequence: 2 givenname: J. surname: Kraus fullname: Kraus, J. – sequence: 3 givenname: M.R. surname: Speicher fullname: Speicher, M.R. – sequence: 4 givenname: M. surname: Oliver-Bonet fullname: Oliver-Bonet, M. – sequence: 5 givenname: V. surname: Murcia fullname: Murcia, V. – sequence: 6 givenname: J. surname: Sarquella fullname: Sarquella, J. – sequence: 7 givenname: J. surname: Egozcue fullname: Egozcue, J. – sequence: 8 givenname: J. surname: Navarro fullname: Navarro, J. – sequence: 9 givenname: J. surname: Benet fullname: Benet, J. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15305051$$D View this record in MEDLINE/PubMed |
| BookMark | eNqF0dtrFDEUB-BoK7ZbffBZkNCHgg-juV8ey9IbFAQv6NuQmTmxU2cmYzID3f71Zt11BRE2LyH8vpyEcxbocAgDIPSKkneUSvue5KWtVOoJWnBODaGUW_MUHVPBRGGk_XaQA0Ok1IZKdbgLDD1Ci5TuCaFGSPUcHVHJiSSSHqOvyzsXXT1BbB_d1IYBB49d1-G7uXcD7l0HOK0GN075M_mE69CPHTxAwtUKp7maoAs9RMD93E3tOioubz5dv0DPvOsSvNzuJ-jL5cXn5XVx--HqZnl-W9RCmKlwSjJvvNVSNMJYo7zgTgrBLEhjvakYZNGA9dzntFLZUpb7QBqhK7D8BJ1t6o4x_JwhTWXfphq6zg0Q5lQqpbUhmu6FjDJtuBb7IbG5j5rvhTTXI8yuK57-A-_DHIfclpIxkR8WxGT0doPqGFKK4Msxtr2Lq5KScj3_cjf_bN9sC85VD81fuR1rBq834IeL3yHuwJ_rp_9Nl1cff4NybDz_BWdWuoY |
| CitedBy_id | crossref_primary_10_1007_s10577_006_1079_9 crossref_primary_10_1093_humrep_del040 crossref_primary_10_3390_ijms22052414 crossref_primary_10_1159_000357152 crossref_primary_10_1093_humrep_del028 crossref_primary_10_1093_humrep_dei023 crossref_primary_10_1093_molehr_gal007 |
| Cites_doi | 10.1126/science.1071220 10.1126/science.274.5287.494 10.1086/340734 10.1093/humupd/6.1.93 10.1007/s004120050340 10.1038/ng0496-368 10.1038/sj.ejhg.5201067 10.1086/302875 10.1086/344714 |
| ContentType | Journal Article |
| Copyright | 2004 S. Karger AG, Basel Copyright 2004 S. Karger AG, Basel Copyright (c) 2004 S. Karger AG, Basel |
| Copyright_xml | – notice: 2004 S. Karger AG, Basel – notice: Copyright 2004 S. Karger AG, Basel – notice: Copyright (c) 2004 S. Karger AG, Basel |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7QP 7TK 7X7 7XB 88A 88E 88I 8AF 8AO 8FD 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P P64 PQEST PQQKQ PQUKI PRINS Q9U RC3 S0X 7TM 7X8 |
| DOI | 10.1159/000079566 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) Calcium & Calcified Tissue Abstracts Neurosciences Abstracts Health & Medical Complete (ProQuest Database) ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) STEM Database ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection AUTh Library subscriptions: ProQuest Central ProQuest Natural Science Collection ProQuest One Community College ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) ProQuest Science Journals ProQuest Biological Science Journals Biotechnology and BioEngineering Abstracts ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic Genetics Abstracts SIRS Editorial Nucleic Acids Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef ProQuest Central Student Technology Research Database ProQuest Central Essentials SIRS Editorial ProQuest Health & Medical Complete (Alumni) ProQuest AP Science ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central Genetics Abstracts Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts ProQuest Central (Alumni) Nucleic Acids Abstracts MEDLINE - Academic |
| DatabaseTitleList | ProQuest Central Student MEDLINE - Academic MEDLINE Genetics Abstracts CrossRef Genetics Abstracts Genetics Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: BENPR name: AUTh Library subscriptions: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISBN | 3318011398 9783318011395 |
| EISSN | 1424-859X |
| EndPage | 21 |
| ExternalDocumentID | 697031711 10_1159_000079566 15305051 79566 |
| Genre | Journal Article |
| GroupedDBID | --- -~X .GJ 0R~ 0~5 0~B 29F 30W 326 34G 36B 39C 3O- 3O. 3V. 4.4 53G 5GY 5RE 7X7 88A 88E 88I 8AF 8AO 8FE 8FH 8FI 8FJ 8UI AAYIC ABJNI ABPAZ ABUWG ACGFO ACGFS ACGOD ACPRK ACPSR ADBBV AENEX AEYAO AFFNX AFJJK AFKRA AHMBA ALDHI ALIPV ALMA_UNASSIGNED_HOLDINGS AZPMC AZQEC BBNVY BENPR BES BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CYUIP DU5 DWQXO E0A EBS EJD EMB EMOBN F5P FB. FYUFA GNUQQ HCIFZ HMCUK HZ~ IH2 IY7 KUZGX L7B LK8 M0L M1P M2P M7P O1H O9- PQQKQ PROAC PSQYO RIG RKO RXVBD S0X SV3 UJ6 UKHRP ZGI CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QP 7TK 7XB 8FD 8FK FR3 K9. P64 PQEST PQUKI PRINS Q9U RC3 7TM 7X8 |
| ID | FETCH-LOGICAL-c448t-a652f8f9754d48986f43a54429e589f8b2e652de9f3f986b68f9120070d47be93 |
| IEDL.DBID | BENPR |
| ISBN | 3805578156 9783805578158 |
| ISSN | 1424-8581 |
| IngestDate | Fri Aug 16 13:23:51 EDT 2024 Sat Aug 17 01:04:22 EDT 2024 Fri Aug 16 20:41:29 EDT 2024 Fri Aug 16 10:01:15 EDT 2024 Thu Oct 10 19:43:08 EDT 2024 Wed Sep 18 12:54:44 EDT 2024 Sat Sep 28 07:41:15 EDT 2024 Thu Aug 29 12:04:30 EDT 2024 Thu Sep 05 20:30:39 EDT 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1-2 |
| Language | English |
| License | Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Copyright 2004 S. Karger AG, Basel |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c448t-a652f8f9754d48986f43a54429e589f8b2e652de9f3f986b68f9120070d47be93 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
| PMID | 15305051 |
| PQID | 224212408 |
| PQPubID | 23462 |
| PageCount | 4 |
| ParticipantIDs | proquest_miscellaneous_17830294 proquest_miscellaneous_21278374 crossref_primary_10_1159_000079566 karger_primary_79566 proquest_miscellaneous_20901873 proquest_miscellaneous_66778071 pubmed_primary_15305051 proquest_journals_224212408 |
| PublicationCentury | 2000 |
| PublicationDate | 2004-01-01 |
| PublicationDateYYYYMMDD | 2004-01-01 |
| PublicationDate_xml | – month: 01 year: 2004 text: 2004-01-01 day: 01 |
| PublicationDecade | 2000 |
| PublicationPlace | Basel, Switzerland |
| PublicationPlace_xml | – name: Basel, Switzerland – name: Switzerland – name: Basel |
| PublicationTitle | Cytogenetic and genome research |
| PublicationTitleAlternate | Cytogenet Genome Res |
| PublicationYear | 2004 |
| Publisher | S. Karger AG |
| Publisher_xml | – name: S. Karger AG |
| References | Hultén MA, Solari AJ, Skakkebaek NE: Abnormal synaptonemal complex in an oligo-chiasmatic man with spermatogenic arrest. Hereditas 78:105-116 (1974). Lynn A, Koehler KE, Judis L, Chan ER, Cherry JP, Schwartz S, Seftel A, Hunt PA, Hassold TJ: Covariation of synaptonemal complex length and mammalian meiotic exchange rates. Science 296:2222-2225 (2002).10.1126/science.1071220 Fauth C, Zhang H, Harabacz S, Brown J, Saracoglu K, Lederer G, Rittinger O, Rost I, Eils R, Kearney L, Speicher MR: A new strategy for the detection of subtelomeric rearrangements. Hum Genet 109:576-583 (2001). Garcia M, Dietrich AJJ, Freixa L, Vink ACG, Ponsà M, Egozcue J: Development of the first meiotic prophase stages in human fetal oocytes observed by light and electron microscopy. Hum Genet 77:223-232 (1987). Schalk JAC, Dietrich AJJ, Vink ACG, Offenberg HH, van Aalderen M, Heyting C: Localization of SCP2 and SCP3 protein molecules within synaptonemal complexes of the rat. Chromosoma 107:540-548 (1998).10.1007/s004120050340 Azofeifa J, Fauth C, Kraus J, Maierhofer C, Langer S, Bolzer A, Reichman J, Schuffenhauer S, Speicher MR: An optimized probe set for the detection of small interchromosomal aberrations by use of 24-color FISH. Am J Hum Genet 66:1684-1688 (2000).1076255210.1086/302875 Barlow AL, Hultén MA: Combined immunocytogenetic and molecular cytogenetic analysis of meiosis I human spermatocytes. Chromosome Res 4:562-573 (1996). Meuwissen RL, Offenberg HH, Dietrich AJ, Riesewijk A, van Iersel M, Heyting C: A coiled-coil related protein specific for synapsed regions of meiotic prophase chromosomes. EMBO J 11:5091-5100 (1992).1464329 Navarro J, Vidal F, Benet J, Templado C, Marina S, Egozcue J: XY-trivalent association and synaptic anomalies in a male carrier of a Robertsonian t(13;14) translocation. Hum Reprod 6:376-381 (1991). Tease C, Hartshorne GM, Hultén MA: Patterns of meiotic recombination in human fetal oocytes. Am J Hum Genet 70:1469-1479 (2002).1199225310.1086/340734 Egozcue S, Blanco J, Vendrell JM, García F, Veiga A, Aran B, Barri PN, Vidal F, Egozcue J: Human male infertility: chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion. Hum Reprod Update 6:93-105 (2000).10.1093/humupd/6.1.93 Froenicke L, Anderson LK, Wienberg J, Ashley T: Male mouse recombination maps for each autosome identified by chromosome painting. Am J Hum Genet 71:1353-1368 (2002).1243249510.1086/344714 Lammers JHM, Offenberg HH, van Aalderen M, Vink ACG, Dietrich AJJ, Heyting C: The gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte-regulated genes. Mol Cell Biol 14:1137-1146 (1994).8289794 Hultén MA, Eliasson R, Tillinger KG: Low chiasma count and other meiotic irregularities in two infertile 46,XY men with spermatogenic arrest. Hereditas 65:285-290 (1970). Oliver-Bonet M, Liehr T, Nietzel A, Heller A, Starke H, Claussen U, Codina-Pascual M, Pujol A, Abad C, Egozcue J, Navarro J, Benet J: Karyotyping of human synaptonemal complexes by cenM-FISH. Eur J Hum Genet 11:879-883 (2003).10.1038/sj.ejhg.5201067 Sun F, Oliver-Bonet M, Liehr T, Starke H, Ko E, Rademaker AW, Navarro J, Benet J, Martin RH: Human male recombination maps for individual chromosomes. Am J Hum Genet 74:521-531 (2004). Schröck E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith MA, Ning Y, Ledbetter DH, Bar-Am J, Soenksen D, Garini Y, Ried T: Multicolor spectral karyotyping of human chromosomes. Science 273:494-497 (1996).866253710.1126/science.274.5287.494 Eijpe M, Heyting C, Gross B, Jessberger R: Association of mammalian SMC1 and SMC3 proteins with meiotic chromosomes and synaptonemal complexes. J Cell Sci 113:673-682 (2000). Langer S, Kraus J, Jentsch I, Speicher MR: Multicolor chromosome painting in diagnostic and research applications. Chromosome Res 12:15-23 (2004). Barlow AL, Hultén MA: Crossing over analysis at pachytene in man. Eur J Hum Genet 6:350-358 (1998).9781043 Speicher MR, Gwyn Ballard S, Ward DC: Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat Genet 12:368-375 (1996).863048910.1038/ng0496-368 Liehr T, Starke H, Weise A, Lehrer H, Claussen U: Multicolor FISH probe sets and their applications. Histol Histopathol 19:229-237 (2004). ref8 ref7 ref9 ref4 ref3 ref6 ref5 ref2 ref1 |
| References_xml | – ident: ref4 doi: 10.1126/science.1071220 – ident: ref7 doi: 10.1126/science.274.5287.494 – ident: ref9 doi: 10.1086/340734 – ident: ref2 doi: 10.1093/humupd/6.1.93 – ident: ref6 doi: 10.1007/s004120050340 – ident: ref8 doi: 10.1038/ng0496-368 – ident: ref5 doi: 10.1038/sj.ejhg.5201067 – ident: ref1 doi: 10.1086/302875 – ident: ref3 doi: 10.1086/344714 |
| SSID | ssj0018456 |
| Score | 1.7743919 |
| Snippet | During meiotic prophase I, homologous chromosomes synapse and recombine. Both events are of vital importance for the success of meiosis. When homologous... |
| SourceID | proquest crossref pubmed karger |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 18 |
| SubjectTerms | Cytogenetic Analysis - methods Humans In Situ Hybridization, Fluorescence - methods Male Original Article Pachytene Stage - genetics Synaptonemal Complex - genetics Telomere - genetics Testis - chemistry Testis - cytology Testis - metabolism |
| Title | Characterization of all human male synaptonemal complexes by subtelomere multiplex-FISH |
| URI | https://karger.com/doi/10.1159/000079566 https://www.ncbi.nlm.nih.gov/pubmed/15305051 https://www.proquest.com/docview/224212408 https://search.proquest.com/docview/17830294 https://search.proquest.com/docview/20901873 https://search.proquest.com/docview/21278374 https://search.proquest.com/docview/66778071 |
| Volume | 107 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LTxsxEB5BAKmXquXRpinUQlwt9mGv7VNVItJQCYR4iNxW3ti-NCRpNpHIv-9411kEUnJce7TaHT9mxjP-PoAzpblGtwM9t0wpyqzQVEUmokNr0JfTiRbKX06-vsn6j-zPgA9CbU4ZyipXe2K1UZvJ0J-RnydV7pJF8uf0H_WkUT65Ghg0tmEnwUAhasHOxeXN7V2TRpCM19eLEkYll3GAFkITXqE2CgwOsjcGafevr7-erXc3K7PT-wQfg79IftUD_Bm27Hgf9moGyeUBPHUbwOX6PiWZOKJHI1Jx75Fn3P5JuRzrqcfcxidSlZDbF1uSYknKRTG3o8mznVmyqix8ob2r-_4hPPYuH7p9GrgS6BADrDnVGU-cdEpwZphUMnMs1ZyhtbFcKieLxKKEscqlDnuLDGVjf04ZGSYKq9IjaI3xS74CkdJ42DejeGqYkh7TTrFhJAuG3opwcRtOVwrLpzUkRl6FElzljVbbcFCrshEJzZ13zd3fd1VPPjUOe1dqz8NSKvNm4Nvwo-nFNeATG3psJ4syj4VHMVNsvUQSKc8-mG6QiD3niNjwjsxj7aFH1oYv9Yx4_XueekLA-NvG7-_Ah7rsx5_ffIfWfLawx-jRzIsT2BYDcRJm738L4fP7 |
| link.rule.ids | 315,783,787,12068,21400,27936,27937,31731,31732,33756,33757,43322,43817,74073,74630 |
| linkProvider | ProQuest |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dT9swED8x2LS9IAb76GDDQnu1lg87sZ8mqOjKBhVioPEWObX9stJ2TSvR_353iRsEEn2MfYqS88fd-c6_H8BXbaRBtwM9t0xrLlxuuI5sxIfOoi9nEpNrupx8Mcj6N-LnrbwNtTlVKKtc7Yn1Rm0nQzoj_5bUuUsRqe_Tf5xIoyi5Ghg0XsAWIVVh7LV1cjq4vGrTCErI5npRIriSKg7QQmjCa9TGHIOD7JFBevmX6q9nz7ubtdnp7cB28BfZcTPAb2HDjXfhVcMgudyDP90WcLm5T8kmnpnRiNXce-wOt39WLcdmSpjb-MTqEnJ37ypWLlm1KOduNLlzM8dWlYX3vHf2u_8Obnqn190-D1wJfIgB1pybTCZeeZ1LYYXSKvMiNVKgtXFSaa_KxKGEddqnHnvLDGVjOqeMrMhLp9P3sDnGL_kITClLsG9Wy9QKrQjTTothpEqB3kru4w4crRRWTBtIjKIOJaQuWq12YK9RZSsSmvefNHd_XNU9xdR67F2pvQhLqSrage_AYduLa4ASG2bsJouqiHNCMdPieYkk0sQ-mK6RiIlzJF_zjoyw9tAj68CHZkY8_L1MiRAw_rT2-w_hdf_64rw4Pxv82oc3TQkQneUcwOZ8tnCf0buZl1_CHP4PC1z2Dg |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fT9swED6xwiZeJja2UbqBhXi1yA87tp8Q6yhlGxViQ-Mtcmr7ZaUtTSvR_37nxM20Se1j7FPkXM6-z77zdwCnSnONsAORW6YUZVZoqiIT0aE1iOV0ooXyl5NvBln_nn194A-BUqgMaZWrNbFaqM1k6M_Iz5IqdskieeZCVsTtl9759In6AlI-0BqqabyAbcGyNGrB9ufLwe1dE1KQjNdXjRJGJZdxoBlCd14xOArcKGT_OKed3z4Xe7YeelYuqLcHrwN2JBf1z34DW3b8Fl7W1SSX-_Cr25Av13crycQRPRqRqg4feURXQMrlWE89_zY-kSqd3D7bkhRLUi6KuR1NHu3MklWW4TPtXf_ov4P73uXPbp-Gugl0iJutOdUZT5x0SnBmmFQycyzVnKHnsVwqJ4vEooSxyqUOe4sMZWN_ZhkZJgqr0vfQGuNIDoBIaTwFnFE8NUxJz2-n2DCSBUPkIlzchpOVwvJpTY-RV9sKrvJGq23Yr1XZiITmzn_N3au7qiefGoe9K7XnYVqVeWMEbThuenE--CCHHtvJosxj4RnNFFsvkUTKVyJMN0jEvv6I2PCOzPPuITprw4faIv5-PU99ccD4cOP4j-EVmm_-_XrwrQO7dTaQP9b5CK35bGE_IdCZF0fBhP8AnRb6PA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterization+of+all+human+male+synaptonemal+complexes+by+subtelomere+multiplex-FISH&rft.jtitle=Cytogenetic+and+genome+research&rft.au=Codina-Pascual%2C+M.&rft.au=Kraus%2C+J.&rft.au=Speicher%2C+M.R.&rft.au=Oliver-Bonet%2C+M.&rft.date=2004-01-01&rft.isbn=3805578156&rft.issn=1424-8581&rft.eissn=1424-859X&rft.volume=107&rft.issue=1-2&rft.spage=18&rft.epage=21&rft_id=info:doi/10.1159%2F000079566&rft_id=info%3Apmid%2F15305051&rft.externalDocID=79566 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1424-8581&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1424-8581&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1424-8581&client=summon |