White shrimp ( Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae

• Published in: Fish & Shellfish Immunology Vol. 20; no. 3; pp. 405 - 408
• Main Authors: de-la-Re-Vega, Enrique, García-Galaz, Alfonso, Díaz-Cinco, Martha E., Sotelo-Mundo, Rogerio R.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2006
• Subjects: Animals; Antibacterial; Gram negative; Litopenaeus vannamei; Lysozyme; Marine; Muramidase - immunology; Muramidase - pharmacology; Nephelometry and Turbidimetry; Penaeidae - immunology; Penaeidae - microbiology; Recombinant protein; Recombinant Proteins - immunology; Recombinant Proteins - pharmacology; Time Factors; Vibrio; Vibrio - drug effects; Vibrio - immunology; Vibrio alginolyticus; Vibrio cholerae; Vibrio parahemolyticus; Mexico; Vibrio parahemolyticus; Vibrio cholerae; Gram negative; Recombinant protein; Antibacterial; Vibrio alginolyticus; Lysozyme
• ISSN: 1050-4648; 1095-9947; 1365-2567
• DOI: 10.1016/j.fsi.2005.06.005

C-type lysozyme has been described as an antibacterial component of the shrimp innate defence system. We determined quantitatively the antibacterial activity of white shrimp ( Litopenaeus vannamei) recombinant lysozyme against three Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae, using a turbidimetric assay with live bacteria and differential bacterial viable count after interaction with the protein. In conclusion, the antibacterial activity of recombinant shrimp lysozyme against Vibrio sp. is at least equal to the values against the Gram positive M. luteus and more active against the shrimp pathogens V. alginolyticus and V. parahemolyticus.