Development and validation of LC/APCI-MS method for the quantification of oat ceramides in skin permeation studies
Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the stratum corneum (SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CE...
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Published in | Analytical and bioanalytical chemistry Vol. 410; no. 20; pp. 4775 - 4785 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.08.2018
Springer Springer Nature B.V |
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Abstract | Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the
stratum corneum
(SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (
Avena abyssinica
) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids.
Graphical abstract
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AbstractList | Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the stratum corneum (SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (Avena abyssinica) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids. Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the stratum corneum (SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat ( Avena abyssinica ) were used as reference standards for the development and validation of the method. The method was linear over the range of 30–1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method’s sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids. Graphical abstract ᅟ Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the stratum corneum (SC), the outer layer of the skin. Skin diseases such as atopic dermatitis, psoriasis, and aged skin are characterized by dysfunctional skin barrier and dryness which are associated with reduced levels of CERs. Replenishing the depleted epidermal CERs with exogenous CERs has been shown to have beneficial effects in improving the skin barrier and hydration. The exogenous CERs such as phyto-derived CERs (PhytoCERs) can be delivered deep into the SC using novel topical formulations. This, however, requires investigating the rate and extent of skin permeation of CERs. In this study, an LC/APCI-MS method to detect and quantify PhytoCERs in different layers of the skin has been developed and validated. The method was used to investigate the skin permeation of PhytoCERs using Franz diffusion cells after applying an amphiphilic cream containing PhytoCERs to the surface of ex vivo human skin. As plant-specific CERs are not commercially available, well-characterized CERs isolated from oat (Avena abyssinica) were used as reference standards for the development and validation of the method. The method was linear over the range of 30-1050 ng/mL and sensitive with limit of detection and quantification of 10 and 30 ng/mL, respectively. The method was also selective, accurate, and precise with minimal matrix effect (with mean matrix factor around 100%). Even if more than 85% of oat CERs in the cream remained in the cream after the incubation periods of 30, 100, and 300 min, it was possible to quantify the small quantities of oat CERs distributed across the SC, epidermis, and dermis of the skin indicating the method's sensitivity. Therefore, the method can be used to investigate the skin permeation of oat CERs from the various pharmaceutical and cosmeceutical products without any interference from the skin constituents such as the epidermal lipids. Graphical abstract ᅟ. |
Audience | Academic |
Author | Frolov, Andrej Wohlrab, Johannes Neubert, Reinhard H. H. Gebre-Mariam, Tsige Tessema, Efrem N. |
Author_xml | – sequence: 1 givenname: Efrem N. surname: Tessema fullname: Tessema, Efrem N. organization: Department of Pharmaceutical Technology and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg – sequence: 2 givenname: Tsige surname: Gebre-Mariam fullname: Gebre-Mariam, Tsige organization: Department of Pharmaceutics and Social Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University – sequence: 3 givenname: Andrej surname: Frolov fullname: Frolov, Andrej organization: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry – sequence: 4 givenname: Johannes surname: Wohlrab fullname: Wohlrab, Johannes organization: Department of Dermatology and Venereology, Medical Faculty, Martin Luther University Halle-Wittenberg, Institute of Applied Dermatopharmacy, Martin Luther University Halle-Wittenberg – sequence: 5 givenname: Reinhard H. H. surname: Neubert fullname: Neubert, Reinhard H. H. email: reinhard.neubert@pharmazie.uni-halle.de organization: Department of Pharmaceutical Technology and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Institute of Applied Dermatopharmacy, Martin Luther University Halle-Wittenberg |
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CitedBy_id | crossref_primary_10_3390_cells11111742 crossref_primary_10_3390_pharmaceutics15122685 crossref_primary_10_1016_j_jpba_2020_113677 crossref_primary_10_1007_s40257_021_00619_2 crossref_primary_10_1016_j_ejpb_2018_02_037 crossref_primary_10_1155_2022_7437905 crossref_primary_10_1016_j_jpba_2020_113373 |
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Keywords | Skin permeability Skin Phyto-derived ceramide LC/APCI-MS Oat ceramide Stratum corneum |
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Snippet | Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the
stratum corneum
(SC), the outer layer of the skin. Skin diseases such as atopic... Ceramides (CERs) are the backbone of the intercellular lipid lamellae of the stratum corneum (SC), the outer layer of the skin. Skin diseases such as atopic... |
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SubjectTerms | Analytical Chemistry Atopic dermatitis Avena - chemistry Biochemistry Ceramides Ceramides - analysis Ceramides - pharmacokinetics Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid - methods Communication Cosmetics industry Cream Dermatitis Dermis Diffusion cells Epidermis Food Science Formulations Glycine max - chemistry Humans Laboratory Medicine Lamellae Limit of Detection Lipids Liquid chromatography Mass spectrometry Mass Spectrometry - methods Measurement Methods Monitoring/Environmental Analysis Nutritional aspects Oats Penetration Psoriasis Skin Skin - metabolism Skin Absorption Skin diseases Stratum corneum |
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Title | Development and validation of LC/APCI-MS method for the quantification of oat ceramides in skin permeation studies |
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